Sui Lumin, Yan Ke, Zhang Huiting, Nie Junyu, Yang Xiaogan, Xu Chang-Long, Liang Xingwei
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, China.
Reproductive Medical Center Nanning Second People's Hospital, Nanning, China.
Front Pharmacol. 2021 Aug 26;12:722779. doi: 10.3389/fphar.2021.722779. eCollection 2021.
Accumulating evidence has demonstrated that benzo(a)pyrene (BaP) exposure adversely affects female reproduction, especially oocyte meiotic maturation and subsequent embryo development. Although we previously found that mogroside V (MV), a major bioactive component of , can protect oocytes from quality deterioration caused by certain stresses, whether MV can alleviate BaP exposure-mediated oocyte meiotic defects remains unknown. In this study, female mice were exposed to BaP and treated concomitantly with MV by gavage. We found that BaP exposure reduced the oocyte maturation rate and blastocyst formation rate, which was associated with increased abnormalities in spindle formation and chromosome alignment, reduced acetylated tubulin levels, damaged actin polymerization and reduced Juno levels, indicating that BaP exposure results in oocyte nucleic and cytoplasmic damage. Interestingly, MV treatment significantly alleviated all the BaP exposure-mediated defects mentioned above, indicating that MV can protect oocytes from BaP exposure-mediated nucleic and cytoplasmic damage. Additionally, BaP exposure increased intracellular ROS levels, meanwhile induced DNA damage and early apoptosis in oocytes, but MV treatment ameliorated these defective parameters, therefore it is possible that MV restored BaP-mediated oocyte defects by reducing oxidative stress. In summary, our findings demonstrate that MV might alleviate oocyte meiotic defects and quality deterioration in BaP-exposed mice.
越来越多的证据表明，接触苯并(a)芘（BaP）会对雌性生殖产生不利影响，尤其是对卵母细胞减数分裂成熟及随后的胚胎发育。尽管我们之前发现罗汉果甜苷V（MV），罗汉果的一种主要生物活性成分，可以保护卵母细胞免受某些应激导致的质量下降影响，但MV是否能减轻BaP暴露介导的卵母细胞减数分裂缺陷仍不清楚。在本研究中，雌性小鼠暴露于BaP并通过灌胃同时给予MV处理。我们发现，BaP暴露降低了卵母细胞成熟率和囊胚形成率，这与纺锤体形成和染色体排列异常增加、乙酰化微管蛋白水平降低、肌动蛋白聚合受损以及Juno水平降低有关，表明BaP暴露导致卵母细胞核酸和细胞质损伤。有趣的是，MV处理显著减轻了上述所有BaP暴露介导的缺陷，表明MV可以保护卵母细胞免受BaP暴露介导的核酸和细胞质损伤。此外，BaP暴露增加了细胞内活性氧水平，同时诱导了卵母细胞中的DNA损伤和早期凋亡，但MV处理改善了这些缺陷参数，因此MV有可能通过降低氧化应激来恢复BaP介导的卵母细胞缺陷。总之，我们的研究结果表明，MV可能减轻BaP暴露小鼠的卵母细胞减数分裂缺陷和质量下降。
