建立用于形成内皮层的具有环烯烃共聚物底物的体外血栓形成试验系统。

Establishment of an in vitro thrombogenicity test system with cyclic olefin copolymer substrate for endothelial layer formation.

作者信息

Lau Skadi, Liu Yue, Maier Anna, Braune Steffen, Gossen Manfred, Neffe Axel T, Lendlein Andreas

机构信息

Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Hereon, Teltow, Germany.

Institute of Chemistry, University of Potsdam, Potsdam, Germany.

出版信息

MRS Commun. 2021;11(5):559-567. doi: 10.1557/s43579-021-00072-6. Epub 2021 Sep 7.

DOI:10.1557/s43579-021-00072-6

PMID:34513262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8422954/

Abstract

UNLABELLED

In vitro thrombogenicity test systems require co-cultivation of endothelial cells and platelets under blood flow-like conditions. Here, a commercially available perfusion system is explored using plasma-treated cyclic olefin copolymer (COC) as a substrate for the endothelial cell layer. COC was characterized prior to endothelialization and co-cultivation with platelets under static or flow conditions. COC exhibits a low roughness and a moderate hydrophilicity. Flow promoted endothelial cell growth and prevented platelet adherence. These findings show the suitability of COC as substrate and the importance of blood flow-like conditions for the assessment of the thrombogenic risk of drugs or cardiovascular implant materials.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1557/s43579-021-00072-6.

摘要

未标记

体外血栓形成测试系统需要在类似血流的条件下将内皮细胞和血小板共同培养。在此，探索了一种市售灌注系统，该系统使用经血浆处理的环烯烃共聚物（COC）作为内皮细胞层的基质。在进行内皮化以及在静态或流动条件下与血小板共同培养之前，对COC进行了表征。COC具有低粗糙度和适度的亲水性。流动促进了内皮细胞生长并防止了血小板黏附。这些发现表明COC作为基质的适用性以及类似血流条件对于评估药物或心血管植入材料血栓形成风险的重要性。