Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo FI-00076, Finland.
CICECO/Department of Chemistry, University of Aveiro, Aveiro 3810-193, Portugal.
J Agric Food Chem. 2021 Sep 22;69(37):10848-10855. doi: 10.1021/acs.jafc.1c04112. Epub 2021 Sep 12.
Although extractives have been symbolized as major bioactive pharmacological compounds from (Salicaceae) bark, we speculated that these pharmaceutical effects cannot be solely attributed to phenolic components and their derivatives, but the long-chain suberin acids also contribute to their therapeutic effects. Hence, isolation and deconstruction of suberin were conducted, for the first time, to enrich our knowledge about the macromolecular components at the cell wall of willow bark. Saponification was adopted to obtain suberin extracts at a yield of approximately 5 wt % based on the bark of the studied hybrids. Gas chromatography-mass spectrometry allowed qualification and quantification of 23 compounds from the released suberin monomers, from which fatty acids represented majority of the isolated suberin, namely, fatty acid methyl esters (C17-C19); mono-carboxylic acid (C7-C16); alpha, omega-dicarboxylic acid (C7-C16); and omega-hydroxy long-chain fatty acids (C16-C22). Additionally, the lipophilic extractive was dominated by piceol, heptacosane, β-sitosterol, and fatty acids (C16-C28) from the studied hybrids. These findings could boost our integrative approach toward full valorization of willow bark.
虽然从（杨柳科）树皮中提取的物质已被符号化为主要的生物活性药理学化合物，但我们推测这些药物作用不能仅归因于酚类成分及其衍生物，而是长链的愈创木脂酸也有助于其治疗效果。因此，首次对愈创木脂进行了分离和解构，以丰富我们对柳树皮细胞壁中高分子成分的了解。采用皂化法，从研究杂交种的树皮中获得了约 5wt%的愈创木脂提取物。气相色谱-质谱联用技术允许从释放的愈创木脂单体中定性和定量 23 种化合物，其中脂肪酸代表分离出的愈创木脂的主要成分，即脂肪酸甲酯（C17-C19）；单羧酸（C7-C16）；α，ω-二羧酸（C7-C16）；和ω-羟基长链脂肪酸（C16-C22）。此外，从研究杂交种中提取的亲脂性提取物主要由扁柏酚、十七烷、β-谷甾醇和脂肪酸（C16-C28）组成。这些发现可以促进我们对柳树皮全面增值的综合方法。
