Department of Chemistry, Yunnan Normal University, Kunming, 650500, PR China.
Department of Chemistry, Yunnan Normal University, Kunming, 650500, PR China.
Talanta. 2021 Dec 1;235:122754. doi: 10.1016/j.talanta.2021.122754. Epub 2021 Jul 30.
Chiral covalent organic frameworks (CCOFs) have potential application in enantioseparation due to their advantages, such as large surface area, abundant chiral recognition sites and good chemical stability in organic solvents. However, the application of CCOFs in high performance liquid chromatography (HPLC) for enantioseparation has been rarely reported because of the shortcomings of CCOFs, such as light weight, irregular shape, and wide particle size distribution. In order to overcome the above shortcomings, a one-pot synthetic method was adopted to prepare a core-shell composite (β-CD-COF@SiO) via the growth of chiral β-CD COF on the surface of amino-functionalized SiO microspheres. The as-prepared β-CD-COF@SiO microspheres were used as a stationary phase for HPLC enantioseparation. The resolution ability of the β-CD-COF@SiO-packed column toward various chiral compounds was investigated using n-hexane/isopropanol as the mobile phase. The results show that the chiral β-CD-COF@SiO-packed column exhibited excellent chiral recognition ability for 24 pairs of chiral compounds with good reproducibility. These successful applications indicate that the preparation of the chiral COFs@SiO core-shell microspheres as a novel stationary phase for enantioseparation has good application prospects in HPLC.
手性共价有机框架（CCOFs）由于其具有大的比表面积、丰富的手性识别位点和在有机溶剂中良好的化学稳定性等优点，在手性分离中具有潜在的应用。然而，由于 CCOFs 存在重量轻、形状不规则、粒径分布较宽等缺点，其在高效液相色谱（HPLC）中的应用很少有报道。为了克服上述缺点，采用一锅法通过在氨基功能化的 SiO2 微球表面生长手性β-CD COF 制备了核壳复合结构（β-CD-COF@SiO）。将制备的β-CD-COF@SiO 微球用作 HPLC 手性分离的固定相。以正己烷/异丙醇为流动相，考察了β-CD-COF@SiO 填充柱对多种手性化合物的拆分能力。结果表明，β-CD-COF@SiO 填充柱对 24 对手性化合物具有良好的手性识别能力和良好的重现性。这些成功的应用表明，制备手性 COFs@SiO 核壳微球作为一种新型的手性分离固定相，在手性 HPLC 中具有良好的应用前景。
