Department of Oncology, Tangshan Central Hospital, Tangshan, China.
Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, China.
Neuroimmunomodulation. 2022;29(1):44-54. doi: 10.1159/000518084. Epub 2021 Sep 10.
Glioma is the most aggressive and malignant type of tumors among primary intracranial tumors. miR-433-3p has been verified to be correlated with the formation and progression of many types of cancers.
In this study, the effects of miR-433-3p and AJUBA on the proliferation, migration, and invasion of glioma and the molecular mechanisms were investigated. We analyzed bioinformatics databases and conducted cell biology experiments to determine that compared with adjacent tissue and normal cells, the expression level of miR-433-3p in glioma tissue and cells was lower, while the expression level of AJUBA was higher. Overexpressing miR-433-3p could significantly inhibit the proliferation, migration, and invasion of glioma cells and promote cell apoptosis.
In addition, after overexpressing miR-433-3p and AJUBA, it was found that overexpressing AJUBA could attenuate the inhibitory effect of overexpressing miR-433-3p on the proliferation, migration, and invasion of glioma cells, which suggested that miR-433-3p regulated the biological function of glioma by downregulating AJUBA expression.
These results proved that miR-433-3p could target to inhibit the expression of AJUBA, thus inhibiting the biological function and malignant progression of glioma.
脑胶质瘤是原发性颅内肿瘤中最具侵袭性和恶性的肿瘤类型。miR-433-3p 已被证实与多种类型癌症的发生和发展相关。
本研究探讨了 miR-433-3p 和 AJUBA 对脑胶质瘤增殖、迁移和侵袭的影响及其分子机制。我们分析了生物信息学数据库并进行了细胞生物学实验，结果表明与邻近组织和正常细胞相比，脑胶质瘤组织和细胞中的 miR-433-3p 表达水平较低，而 AJUBA 的表达水平较高。过表达 miR-433-3p 可显著抑制脑胶质瘤细胞的增殖、迁移和侵袭，并促进细胞凋亡。
此外，过表达 miR-433-3p 和 AJUBA 后发现，过表达 AJUBA 可减弱过表达 miR-433-3p 对脑胶质瘤细胞增殖、迁移和侵袭的抑制作用，表明 miR-433-3p 通过下调 AJUBA 表达来调节脑胶质瘤的生物学功能。
这些结果证明 miR-433-3p 可以靶向抑制 AJUBA 的表达，从而抑制脑胶质瘤的生物学功能和恶性进展。
