在生物反应器中使用微载体片大规模扩增脐带间充质干细胞。
Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor.
机构信息
Beijing CytoNiche Biotechnology Co., Ltd., Beijing, China.
Department of Biomedical Engineering, School of Medicine, Tsinghua-PKU Center for Life Sciences, Tsinghua University, Beijing, China.
出版信息
Methods Mol Biol. 2022;2436:113-125. doi: 10.1007/7651_2021_425.
Mesenchymal stem cells show great potential in tissue engineering and cell-based therapies. This protocol demonstrates the use of 3D TableTrix microcarrier tablets for large-scale manufacturing of human umbilical cord mesenchymal stem cells (hUCMSCs) in a 5-L stirred tank bioreactor. 3D TableTrix microcarrier tablets readily disperse into tens of thousands of porous microcarriers to simplify cell seeding, and 3D FloTrix vivaSPIN bioreactor could automate, monitor, and control the entire culture process. 3D TableTrix microcarriers could also be fully dissolved upon adding dissolution reagent to gently harvest the expanded cells at a high recovery rate. With this protocol, more than 10 cells could be produced in a 5-L bioreactor.
间充质干细胞在组织工程和基于细胞的治疗中具有巨大的潜力。本方案展示了使用 3D TableTrix 微载体片在 5L 搅拌罐生物反应器中大规模制造人脐带间充质干细胞（hUCMSCs）的方法。3D TableTrix 微载体片易于分散成数以万计的多孔微载体，从而简化细胞接种，3D FloTrix vivaSPIN 生物反应器可以实现整个培养过程的自动化、监测和控制。添加溶解试剂后，3D TableTrix 微载体可以完全溶解，从而以高回收率温和地收获扩增的细胞。使用本方案，可在 5L 生物反应器中生产超过 10 亿个细胞。
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