Shanghai Key Lab of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice, China; Department of Legal Medicine, College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China.
Department of Legal Medicine, College of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China.
Leg Med (Tokyo). 2021 Nov;53:101964. doi: 10.1016/j.legalmed.2021.101964. Epub 2021 Sep 8.
In this study, sensitive analytical procedure for detection and quantification of etaqualone in human hair samples using gas chromatography tandem mass spectrometry (GC-MS/MS) was newly established, and applied it to authentic human samples obtained from an abuser. In this method, the hair samples were treated with hydrochloric acid and then extracted with ethyl ether. The ether layer was dried in a warm water bath, and the residue was reconstituted in ethyl acetate, followed by GC-MS/MS analysis. Multiple reaction monitoring (MRM) mode was used for data collection, and quantitative analysis was performed using internal standard method. Good linear relationship within the concentration range of 1-100 pg/mg were obtained in calibrators for the hair samples showing its correlation coefficient value was 0.9993. The lower limit of quantitation in this study was 1 pg/mg and the recovery rate examined ranged from 100.4% to 108.5%. The intra-day precision and accuracy were less than 5.0% and 5.8%, respectively. The inter-day precision and accuracy were lower than 6.4% and 4.6%, respectively. Using this established method, etaqualone could be detected in the hair sample obtained from a suspected user to be level of 65.2 pg/mg. It should be expected that the method established in this study would contribute to rapid detection and identification of psychotropic drug etaqualone among multiple fields including forensic investigation, clinical application and of course public health matters.
在这项研究中，建立了一种新的使用气相色谱串联质谱（GC-MS/MS）检测和定量人发中依他喹酮的灵敏分析方法，并将其应用于从滥用者中获得的真实人体样本。在该方法中，毛发样品先用盐酸处理，然后用乙醚提取。乙醚层在温水浴中干燥，残渣用乙酸乙酯重新溶解，然后进行 GC-MS/MS 分析。采用多反应监测（MRM）模式进行数据采集，并用内标法进行定量分析。校准品在 1-100 pg/mg 浓度范围内呈良好的线性关系，其相关系数值为 0.9993。本研究的定量下限为 1 pg/mg，回收率范围为 100.4%至 108.5%。日内精密度和准确度分别小于 5.0%和 5.8%。日间精密度和准确度分别低于 6.4%和 4.6%。使用该建立的方法，可以在从疑似使用者获得的毛发样本中检测到依他喹酮，其浓度为 65.2 pg/mg。可以预期，本研究建立的方法将有助于在法医调查、临床应用以及当然还有公共卫生等多个领域快速检测和识别精神药物依他喹酮。
