Prevalence and Multiplicity of Thrombophilia Genetic Polymorphisms of , , , and : A Cross-Sectional Study on a Healthy Jordanian Population.

BACKGROUND

, , , and are the most common genes associated with thrombophilia genetic variants, which vary among different populations and ethnic groups. Little is known about the prevalence and multiplicity of these variants in Jordan. The aim of this study was to estimate the prevalence and multiplicity of the G1691A, H1299R, 1298A>C, 677C>T, 20210G>A, and 675 4G/5G variants among healthy Jordanians.

METHODS

This cross-sectional study was conducted on randomly selected healthy Jordanian participants. Non-Jordanians and those with a history of arterial/venous thrombosis, atherosclerosis, or a history of recurrent abortions were excluded from the study. PCR was used to detect variants in DNA extracted from participants' blood samples.

RESULTS

A total of 300 subjects were screened: 170 (56.7%) females with an average age of 27.78±9.32 years and 130 (43.3%) males with an average age of 29.88±8.55 years. Genetic variants (at least one) were found in 75% of the subjects (81.2% among females and 66.9% among men), while 64.7%, 52%, and 12% were found to have at least two, three, and four variants, respectively. Overall, 21%, 29%, 54.3%, 27.3%, 7.7%, and 66% of participants were found to have G1691A, H1299R, 1298A>C, 677C>T, 20210G>A, and 675 4G/5G gene variants, respectively.

CONCLUSION

Three-quarters of our population had at least one of the thrombophilia genetic variants, and most had more than one variant. The most common variants detected were associated with , followed by , , and then . We observed that females had higher prevalence estimates than males. However, multiplicity among males was significantly higher than females. Our findings indicated noticeable differences in prevalence estimates compared with other populations.