Zheng Qiuyue, Ji Chao, Liu Ran, Xu Junyi, Wang Yong, Yang Aifu, Zheng Wenjie, Cao Jijuan
Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, 116600, China.
Laboratory for Quality Control and Traceability of Food, Tianjin Normal University, Tianjin, 300387, People's Republic of China.
Anal Bioanal Chem. 2021 Nov;413(26):6671-6676. doi: 10.1007/s00216-021-03634-8. Epub 2021 Sep 14.
Polymerase chain reaction (PCR) technology has become a standard technique for the detection of genetically modified organisms (GMOs). However, this method requires a PCR amplification process which is both expensive and time-consuming. Herein, we propose electric field-induced release and measurement (EFIRM) technology as an alternative method for GMO screening. The specificity and sensitivity of the EFIRM assay were proven to be comparable to those of the real-time PCR method for detecting genetically modified soybeans. After all the parameters had been evaluated, the actual evaluation of soybean samples from soybean cargoes was performed. An actual EFIRM screening was performed on 157 soybean cargo samples, which had 102 transgenic soybean samples containing the GTS-40-3-2 gene, through a blind trial at the Dalian port of China. Our results showed that 101 transgenic soybean samples were correctly detected, with only one false-negative case, and 55 non-transgenic soybean samples were detected as negative; this demonstrates that the EFIRM assay is an effective, accurate, simple, and economical novel method for detecting transgenic products, which may have a positive impact on the development of rapid on-site GMO monitoring platforms.
聚合酶链反应（PCR）技术已成为检测转基因生物（GMO）的标准技术。然而，该方法需要进行PCR扩增过程，这既昂贵又耗时。在此，我们提出电场诱导释放与测量（EFIRM）技术作为转基因生物筛选的替代方法。EFIRM检测的特异性和灵敏度已被证明与检测转基因大豆的实时PCR方法相当。在评估了所有参数后，对来自大豆货物的大豆样品进行了实际评估。通过在中国大连港进行的盲测，对157个大豆货物样品进行了实际的EFIRM筛选，其中有102个含有GTS-40-3-2基因的转基因大豆样品。我们的结果表明，正确检测出101个转基因大豆样品，仅出现1例假阴性情况，55个非转基因大豆样品被检测为阴性；这表明EFIRM检测是一种有效、准确、简单且经济的检测转基因产品的新方法，可能对快速现场转基因生物监测平台的发展产生积极影响。
