Optics and Biosciences Laboratory, CNRS, INSERM, Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau, France.
Chirality. 2021 Nov;33(11):747-757. doi: 10.1002/chir.23359. Epub 2021 Sep 14.
Time-resolved circular dichroism has been developed for a few decades to investigate rapid conformational changes in (bio)molecules. In our group, we have come up with several experimental set-ups allowing us to study pico-nanosecond local phenomena in molecular systems as well as much slower effects occurring in proteins and DNA in the folding processes. After an overview of the worldwide realizations in this domain, we present emblematic experiments that we have carried out, spanning time domain from picoseconds to seconds.
时间分辨圆二色性已经发展了几十年，用于研究（生物）分子中的快速构象变化。在我们的小组中，我们提出了几种实验方案，使我们能够研究分子系统中的皮秒纳秒级局部现象以及蛋白质和 DNA 折叠过程中发生的较慢效应。在概述了该领域的全球实现情况之后，我们展示了一些具有代表性的实验，这些实验涵盖了从皮秒到秒的时间域。
