使用柔性有机电化学晶体管对新冠病毒免疫球蛋白G进行超快速、灵敏且便携的检测。
Ultrafast, sensitive, and portable detection of COVID-19 IgG using flexible organic electrochemical transistors.
作者信息
Liu Hong, Yang Anneng, Song Jiajun, Wang Naixiang, Lam Puiyiu, Li Yuenling, Law Helen Ka-Wai, Yan Feng
机构信息
Department of Applied Physics, The Hong Kong Polytechnic University, Hung Hom, 999077 Kowloon, Hong Kong.
Department of Health Technology and Informatics Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hung Hom, 999077 Kowloon, Hong Kong.
出版信息
Sci Adv. 2021 Sep 17;7(38):eabg8387. doi: 10.1126/sciadv.abg8387. Epub 2021 Sep 15.
The outbreak of COVID-19 and its continued spread have seriously threatened public health. Antibody testing is essential for infection diagnosis, seroepidemiological analysis, and vaccine evaluation. However, convenient, fast, and accurate antibody detection remains a challenge in this protracted battle. Here, we report an ultrafast, low-cost, label-free, and portable SARS-CoV-2 immunoglobulin G (IgG) detection platform based on organic electrochemical transistors (OECTs), which can be remotely controlled by a mobile phone. To enable faster detection, voltage pulses are applied on the gate electrode of the OECT to accelerate binding between the antibody and antigen. By optimizing ion concentrations and pH values of test solutions, we realize specific detection of SARS-CoV-2 IgG in several minutes with a detectable region from 10 fM to 100 nM, which encompasses the range of serum SARS-CoV-2 IgG levels in humans. These portable sensors show promise for use in diagnosis and prognosis of COVID-19.
新型冠状病毒肺炎（COVID-19）的爆发及其持续传播严重威胁了公众健康。抗体检测对于感染诊断、血清流行病学分析和疫苗评估至关重要。然而，在这场持久战中，便捷、快速且准确的抗体检测仍然是一项挑战。在此，我们报告了一种基于有机电化学晶体管（OECT）的超快速、低成本、无标记且便携式的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）免疫球蛋白G（IgG）检测平台，该平台可由手机远程控制。为实现更快检测，在OECT的栅电极上施加电压脉冲以加速抗体与抗原之间的结合。通过优化测试溶液的离子浓度和pH值，我们在几分钟内实现了对SARS-CoV-2 IgG的特异性检测，可检测范围为10 fM至100 nM，涵盖了人类血清中SARS-CoV-2 IgG水平的范围。这些便携式传感器在COVID-19的诊断和预后方面显示出应用前景。
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