Ackermans Mariëtte T
Endocrine Laboratory, Department of Clinical Chemistry, Amsterdam UMC, University of Amsterdam, the Netherlands.
Pract Lab Med. 2021 Sep 1;27:e00254. doi: 10.1016/j.plabm.2021.e00254. eCollection 2021 Nov.
Sensitivity is often an issue in bioanalytical LC-MS/MS applications. Commonly investigated parameters to improve it include additives to mobile phase, derivatization and sample-preparation. The nature of the column, however, is not frequently evaluated.
The sensitivity is compared for 18 different reversed phase and 2 different HILIC columns using 2 different mobile phase compositions. Sensitivity was evaluated in terms of S/N for 1,5 pg oxytocin on column, using a scouting gradient.
The measured signal to noise ranged from 55 to 1473, indicating a substantial difference in sensitivity. The most sensitive columns were the Synergi Hydro RP (reversed phase) and the Atlantis HILIC (HILIC).
This study shows that choosing the right column contributes to the sensitivity of the method.
在生物分析液相色谱-串联质谱（LC-MS/MS）应用中，灵敏度常常是一个问题。常用于提高灵敏度的参数包括流动相添加剂、衍生化和样品制备。然而，色谱柱的性质却较少被评估。
使用两种不同的流动相组成，比较18种不同的反相色谱柱和2种不同的亲水作用色谱（HILIC）柱的灵敏度。采用探索性梯度，根据柱上1.5 pg催产素的信噪比（S/N）评估灵敏度。
测得的信噪比范围为55至1473，表明灵敏度存在显著差异。最灵敏的色谱柱是Synergi Hydro RP（反相）柱和Atlantis HILIC（HILIC）柱。
本研究表明，选择合适的色谱柱有助于提高方法的灵敏度。
