Malemud C J, Goldberg V M, Miller L M
Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Biochem Int. 1987 Jun;14(6):987-95.
Primary and first passage rabbit chondrocyte cultures synthesized a "free" form of hyaluronic acid (HA-f) previously characterized in rabbit cartilage. HA-f was isolated from the [3H] glcN/35SO4-labelled cell-associated-fraction (CAF) and from the culture medium by successive equilibrium centrifugations in Cs2SO4/CsCl/Cs2SO4 under low salt conditions. The culture medium HA-f appeared in the void volume of Sepharose CL-2B eluted with low salt, (0.5M sodium acetate), and was susceptible to digestion with Streptomyces hyaluronidase. HA-f aggregated purified rabbit cartilage proteoglycan monomer. These results indicated that HA-f probably subserves hyaluronic acid already complexed with proteoglycan monomer. Newly synthesized HA-f may be required for the continual formation of proteoglycan aggregates.
原代和传代一次的兔软骨细胞培养物合成了一种先前在兔软骨中鉴定出的“游离”形式的透明质酸（HA-f）。HA-f通过在低盐条件下于Cs2SO4/CsCl/Cs2SO4中连续平衡离心，从[3H]葡糖胺/35SO4标记的细胞相关部分（CAF）和培养基中分离出来。培养基中的HA-f出现在用低盐（0.5M醋酸钠）洗脱的琼脂糖CL-2B的空体积中，并且易被透明质酸酶消化。HA-f使纯化的兔软骨蛋白聚糖单体聚集。这些结果表明，HA-f可能为已经与蛋白聚糖单体复合的透明质酸发挥作用。新合成的HA-f可能是蛋白聚糖聚集体持续形成所必需的。
