兔细胞色素P450IIC4 cDNA的特性及苯巴比妥对相关肝mRNA水平的诱导作用。

Characterization of rabbit cytochrome P450IIC4 cDNA and induction by phenobarbital of related hepatic mRNA levels.

作者信息

Zhao J, Leighton J K, Kemper B

出版信息

Biochem Biophys Res Commun. 1987 Jul 15;146(1):224-31. doi: 10.1016/0006-291x(87)90714-5.

Abstract

We have cloned cDNA containing a partial sequence of a rabbit cytochrome P-450 (designated cytochrome P450IIC4) cDNA that is a member of the cytochrome P450IIC subfamily. The cDNA contains 770 bp of which the first 429 code for the C-terminal 143 amino acids of cytochrome P450IIC4. The protein coding region of the cDNA is 98% homologous with that of cytochrome P450IIC5 and the 3' untranslated region is about 90% homologous. In contrast to the constitutive isozyme, cytochrome P450IIC5, mRNA in the liver that hybridized to the 3' untranslated region of cytochrome P450IIC4 cDNA was increased about 8-fold 24 hours after a single injection of phenobarbital.

摘要

我们克隆了包含兔细胞色素P-450（命名为细胞色素P450IIC4）cDNA部分序列的cDNA，细胞色素P450IIC4是细胞色素P450IIC亚家族的成员。该cDNA含有770bp，其中前429bp编码细胞色素P450IIC4的C末端143个氨基酸。该cDNA的蛋白质编码区与细胞色素P450IIC5的蛋白质编码区有98%的同源性，其3'非翻译区约有90%的同源性。与组成型同工酶细胞色素P450IIC5不同，单次注射苯巴比妥24小时后，肝脏中与细胞色素P450IIC4 cDNA的3'非翻译区杂交的mRNA增加了约8倍。