Department of Prosthodontics, Faculty of Dentistry, Istanbul University, Istanbul, Turkey.
Clinic of Chest Diseases, Sureyyapasa Chest Diseases and Chest Surgery Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.
Niger J Clin Pract. 2021 Sep;24(9):1313-1320. doi: 10.4103/njcp.njcp_590_20.
Tuberculosis (TB) is a communicable disease as well as an airborne disease. Mycobacterium tuberculosis (MTB) could survive on dental materials shipped to dental laboratories.
The aim of this study was to determine the number of bacilli held on the prosthetic material and the effect of chemical disinfection agents on various prosthetic materials that were shipped to dental laboratory of TB patient.
The study consisted of three study groups, and a control group. 10 mm x 2 mm disc-shaped (n = 18 for each group, n = 72 in total) nickel-chromium alloy (Ni-Cr), polymethylmethacrylate (PMMA), and dental ceramic (DC) samples were prepared. After exposure to MTB 24 hours in a 37°C incubator, six samples for each group (PMMA), Ni-Cr alloy and a control group DC samples) were exposed to three disinfectants; 10 minutes into 2% glutaraldehyde, 10 minutes into 5% sodium hypochlorite, and 1 minute into alcohol-based disinfectant after vortexed in distilled water. Colony forming units (CFU/ml) were calculated per milliliters. Two-way ANOVA statistical analysis method was used, and a P value less than 0.05 was considered as significant.
The bacteria count for six Ni-Cr alloy disc-shaped specimens were recorded as 40, 10, 8, 6, 5, and 4 CFU/ml, respectively. Intensity of the colonies were found to be lower in other groups. 5 CFU/ml were detected on a single PMMA sample in the control group, and 40 CFU/ml were detected on one of the dental ceramic sample. No MTB uptake was observed on any sample in the 2% glutaraldehyde and 5% NaOCl disinfectant study groups. In alcohol-based disinfectant group, 1 CFU/ml was observed on Ni-Cr alloy sample. The effect of prosthetic materials used in this experimental study were not statistically significant on the CFU (p = 0.293). However, the disinfectants use was statistically significant on the number of colonies (p = 0.004).
NaOCl and glutaraldehyde appeared to be more effective than alcohol-based disinfectant in removing MTB from Ni-Cr alloy, PMMA and dental ceramic surfaces.
结核病（TB）是一种传染性疾病，也是一种空气传播疾病。结核分枝杆菌（MTB）可以在运往牙科实验室的牙科材料上存活。
本研究旨在确定在运往结核患者牙科实验室的义齿材料上保持的杆菌数量以及各种化学消毒剂对各种义齿材料的影响。
本研究包括三个研究组和一个对照组。制备 10mmx2mm 盘状（每组 18 个，共 72 个）镍铬合金（Ni-Cr）、聚甲基丙烯酸甲酯（PMMA）和牙科陶瓷（DC）样本。将样本在 37°C 孵育箱中暴露于 MTB 24 小时后，将每组的 6 个样本（PMMA）、Ni-Cr 合金和对照组 DC 样本）暴露于三种消毒剂中；2%戊二醛浸泡 10 分钟、5%次氯酸钠浸泡 10 分钟、在蒸馏水中涡旋 1 分钟后使用酒精基消毒剂。计算每毫升的菌落形成单位（CFU/ml）。使用双向方差分析方法，P 值小于 0.05 被认为具有统计学意义。
六个 Ni-Cr 合金盘状样本的细菌计数分别记录为 40、10、8、6、5 和 4CFU/ml，其他组的菌落强度较低。对照组单个 PMMA 样本中检测到 5CFU/ml，单个牙科陶瓷样本中检测到 40CFU/ml。在 2%戊二醛和 5%次氯酸钠消毒剂研究组的任何样本中均未观察到 MTB 吸收。在酒精基消毒剂组中，Ni-Cr 合金样本中观察到 1CFU/ml。本实验研究中使用的义齿材料对 CFU 没有统计学意义（p=0.293）。然而，消毒剂的使用对菌落数量有统计学意义（p=0.004）。
与酒精基消毒剂相比，次氯酸钠和戊二醛似乎更能有效从 Ni-Cr 合金、PMMA 和牙科陶瓷表面去除 MTB。
