Gao Cheng, Shen Jianbo, Chen Weipeng, Yao Lanqing, Liang Xiaoliang, Zhu Renfei, Chen Zhong
Department of General Surgery, Affiliated Hospital of Nantong University, Nantong, China.
Medical College of Nantong University, Nantong, China.
J Gastrointest Oncol. 2021 Aug;12(4):1905-1926. doi: 10.21037/jgo-21-390.
Liver cancer is one of the major causes of cancer death worldwide, incurring high mortality and a significant financial burden on the healthcare system. Abnormal RNA-binding proteins (RBPs) have been found to be associated with carcinogenesis in liver cancer. Among these, RNA-binding motif protein 12 () is located in the exon junction complex (EJC). The goal of this study was to determine what role plays in hepatocellular carcinoma (HCC) from a biological perspective.
The Tumor IMmune Estimation Resource (TIMER) and the Human Protein Atlas database were used to examine the expression level of , with the UALCAN and Gene Expression Profiling Interactive Analysis (GEPIA) databases used to investigate the relationship between and other noteworthy clinical features. expression in cells and tissue samples was detected using quantitative real-time polymerase chain reaction (qRT-PCR) and western blot analysis. The functional network of in HCC was studied using LinkedOmics and gene set enrichment analysis (GSEA), while the effects of hypomethylation on the expression of in HCC was investigated using methylation databases. Finally, we used TIMER and CIBERSORT to investigate the relationship between immune cell infiltration and in HCC.
is highly elevated in HCC tissues and cells, and it can be used to predict the prognosis of patients with HCC. Analysis with LinkedOmics and GSEA revealed to be closely linked with tumor progression. Furthermore, hypomethylation was linked to an increase in expression in HCC, while was associated with immune cell infiltration.
This study shows that an elevated level of in HCC indicates a poor patient prognosis. Furthermore, according to LinkedOmics and GSEA analyses, was implicated in the most important hallmark pathways. Our findings suggest that overexpression is caused by hypomethylation and that plays a key role in liver cancer tumor immunity.
肝癌是全球癌症死亡的主要原因之一，死亡率高，给医疗系统带来巨大经济负担。已发现异常的RNA结合蛋白（RBP）与肝癌的致癌作用有关。其中，RNA结合基序蛋白12（RBM12）位于外显子连接复合体（EJC）中。本研究的目的是从生物学角度确定RBM12在肝细胞癌（HCC）中发挥的作用。
使用肿瘤免疫估计资源（TIMER）和人类蛋白质图谱数据库检测RBM12的表达水平，使用UALCAN和基因表达谱交互分析（GEPIA）数据库研究RBM12与其他值得注意的临床特征之间的关系。采用定量实时聚合酶链反应（qRT-PCR）和蛋白质免疫印迹分析检测细胞和组织样本中RBM12的表达。使用LinkedOmics和基因集富集分析（GSEA）研究RBM12在HCC中的功能网络，同时使用甲基化数据库研究低甲基化对HCC中RBM12表达的影响。最后，我们使用TIMER和CIBERSORT研究免疫细胞浸润与HCC中RBM12的关系。
RBM12在HCC组织和细胞中高度升高，可用于预测HCC患者的预后。LinkedOmics和GSEA分析显示，RBM12与肿瘤进展密切相关。此外，低甲基化与HCC中RBM12表达增加有关，而RBM12与免疫细胞浸润有关。
本研究表明，HCC中RBM12水平升高表明患者预后不良。此外，根据LinkedOmics和GSEA分析，RBM12参与了最重要的标志性通路。我们的研究结果表明，RBM12过表达是由低甲基化引起的，并且RBM12在肝癌肿瘤免疫中起关键作用。
