A.V. Zhirmunsky Institute of Marine Biology, National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences (NSCMB FEB RAS), Vladivostok, Russia.
Il'ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences (POI FEB RAS), Vladivostok, Russia.
Environ Sci Pollut Res Int. 2022 Feb;29(8):11281-11290. doi: 10.1007/s11356-021-16487-6. Epub 2021 Sep 17.
Micro- and nano-sized particles of polytetrafluoroethylene (PTFE) were used as model (reference) particles to study the biological effects of plastic pollution. Since the PTFE molecule contains fluorine, considered as an "atomic marker" sharply distinguishing it from other common plastics, micro- and nano-particles of PTFE have a specific crystalline structure and are, therefore, well identified by the methods of polarized light microscopy (POL), Raman microspectroscopy (micro-Raman), and energy-dispersive spectroscopy (EDS). Examples of PTFE particles detection in hemolimph of the cockroach Blatella germanica, in hemolimph of the larva and in faecal pellets of imago of a fly Lucilia sp., in the stomach and hingat of brine shrimp Artemia salina, and in association with cell wall of green unicellular alga Chlorococcus sp. are provided. The presented results strongly suggest that PTFE particles can be detected and identified in the biological medium using the method of "atomic markers", polarization microscopy and Raman spectroscopy.
聚四氟乙烯（PTFE）的微纳米颗粒被用作模型（参考）颗粒，以研究塑料污染的生物效应。由于 PTFE 分子含有氟，被认为是一种“原子标记”，可以将其与其他常见塑料明显区分开来，因此 PTFE 的微纳米颗粒具有特定的晶体结构，并且可以通过偏光显微镜（POL）、拉曼微光谱（micro-Raman）和能谱（EDS）等方法很好地识别。提供了在德国蟑螂 Blatella germanica 的血淋巴、幼虫的血淋巴和成虫的粪便颗粒、盐水虾 Artemia salina 的胃和铰链以及绿单细胞藻类 Chlorococcus sp. 的细胞壁中检测到 PTFE 颗粒的例子。所呈现的结果强烈表明，可以使用“原子标记”、偏光显微镜和拉曼光谱的方法在生物介质中检测和识别 PTFE 颗粒。
