Xiangya School of Medicine, 47904Central South University, China.
Department of Anesthesiology, 56663Guizhou Provincial People's Hospital, The Affiliated Hospital of Guizhou University, China.
Sci Prog. 2021 Jul-Sep;104(3):368504211043763. doi: 10.1177/00368504211043763.
Numerous studies have demonstrated that anesthetics' exposure to neonates imposes toxicity on the developing brain but the underlying mechanisms need to be further elucidated. Our present study aimed to explore the role of small conductance Ca-activated potassium channel type2 in memory and learning dysfunction caused by exposing neonates to sevoflurane. Postnatal day 7 Sprague-Dawley rats and hemagglutinin-tagged small conductance Ca-activated potassium channel type2 channel transfected COS-7 cells were exposed to sevoflurane and the trafficking of small conductance Ca-activated potassium channel type2 channels was analyzed; furthermore, memory and learning ability was analyzed by the Morris water maze test on postnatal day30-35 (juvenile period). Our results showed that sevoflurane exposure inhibited small conductance Ca-activated potassium channel type2 channel endocytosis in both hippocampi of postnatal day 7 rats and hemagglutinin-tagged small conductance Ca-activated potassium channel type2 channel transfected COS-7 cells and the memory and learning ability was impaired in the juvenile period after sevoflurane exposure to neonatal rats. Herein, our results demonstrated that exposing neonates to sevoflurane caused memory and learning impairment via dysregulating small conductance Ca-activated potassium channel type2 channels endocytosis.
大量研究表明，麻醉剂暴露于新生儿会对发育中的大脑造成毒性，但潜在机制仍需进一步阐明。本研究旨在探讨小电导钙激活钾通道 2 型（small conductance Ca-activated potassium channel type2，SK2）在七氟醚暴露致新生儿记忆和学习功能障碍中的作用。在新生后第 7 天的 Sprague-Dawley 大鼠和转染有血影蛋白标记的 SK2 通道的 COS-7 细胞中，我们暴露于七氟醚，并分析 SK2 通道的转运情况；此外，通过 Morris 水迷宫测试分析了新生后第 30-35 天（青少年期）的记忆和学习能力。我们的结果表明，七氟醚暴露抑制了新生后第 7 天大鼠双侧海马和转染有血影蛋白标记的 SK2 通道的 COS-7 细胞中 SK2 通道的内吞作用，并且在七氟醚暴露于新生大鼠后，青少年期的记忆和学习能力受损。因此，我们的研究结果表明，七氟醚暴露通过调节 SK2 通道的内吞作用导致新生儿记忆和学习功能障碍。
