硒通过调节 ROS 诱导的 NLRP3 炎性小体缓解金黄色葡萄球菌诱导的奶牛乳腺上皮细胞炎症。
Selenium Ameliorates S. aureus-Induced Inflammation in Bovine Mammary Epithelial Cells by Regulating ROS-Induced NLRP3 Inflammasome.
机构信息
Linyi Academy of Agricultural Sciences, Linyi, 276012, China.
College of Agriculture and Forestry Science, Linyi University, Linyi, 276005, China.
出版信息
Biol Trace Elem Res. 2022 Jul;200(7):3171-3175. doi: 10.1007/s12011-021-02924-7. Epub 2021 Sep 17.
In this study, the regulation effects of selenium (Se) on the expression of pyrin domain-containing protein (NLRP) 3 inflammasome and reactive oxygen species (ROS) in bovine mammary epithelial cells (bMECs) infected by Staphylococcus aureus (S. aureus) were detected. bMECs were treated with 8 μmol/L NaSeO for 12 h before infection with S. aureus for 2 h. Through flow cytometry, Western blot, and qRT-PCR analysis, the expression of ROS and NLRP3 imflammasome was detected. Results showed Se significantly reduced the ROS level in bMECs; at the same time, the expressions of NLRP3, ASC, caspase-1, Pro-IL-1β, and IL-1β were also decreased. In conclusion, Se inhibits S. aureus-induced inflammation by suppressing the activation of NLRP3 inflammasome and ROS in bMECs.
本研究检测了硒（Se）对金黄色葡萄球菌（S. aureus）感染奶牛乳腺上皮细胞（bMECs）后吡咯啉结构域包含蛋白（NLRP）3 炎性小体和活性氧（ROS）表达的调节作用。bMECs 在感染 S. aureus 前用 8μmol/L NaSeO 处理 12 h。通过流式细胞术、Western blot 和 qRT-PCR 分析检测 ROS 和 NLRP3 炎性小体的表达。结果表明，Se 显著降低了 bMECs 中的 ROS 水平；同时，NLRP3、ASC、caspase-1、Pro-IL-1β 和 IL-1β 的表达也降低。综上所述，Se 通过抑制 bMECs 中 NLRP3 炎性小体和 ROS 的激活来抑制 S. aureus 诱导的炎症。
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