Universidad Nacional de Río Cuarto, FCEFQyN, Departamento de Biología Molecular, Río Cuarto, Córdoba, Argentina; CONICET, Universidad Nacional de Río Cuarto, Instituto de Biotecnología Ambiental y Salud, (INBIAS), Río Cuarto, Córdoba, Argentina.
Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Córdoba, Argentina; CONICET, Universidad Nacional de Córdoba, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Córdoba, Argentina.
Plant Physiol Biochem. 2021 Oct;167:862-873. doi: 10.1016/j.plaphy.2021.09.005. Epub 2021 Sep 8.
In plants, lipid metabolism and remodelling are key mechanisms for survival under temperature stress. The present study attempted to compare the lipid profile in barley roots both under chilling stress treatment and in the subsequent recovery to stress. Lipids were obtained through a single-extraction method with a polar solvent mixture, followed by mass spectrometry analysis. The results indicate that lipid metabolism was significantly affected by chilling. Most of the glycerolipids analysed returned to control values during short- and long-term recovery, whereas several representative phosphatidic acid (PA) molecular species were edited during long-term recovery. Most of the PA molecular species that increased in the long-term had the same acyl chains as the phosphatidylcholine (PC) species that decreased. C34:2 and C36:4 underwent the most remarkable changes. Given that the mechanisms underlying the acyl-editing of PC in barley roots remain elusive, we also evaluated the contribution of lysophosphatidylcholine acyltransferases (HvLPCAT) and phospholipase A (HvPLA). In line with the aforementioned results, the expression of the HvLPCAT and HvPLA genes was up-regulated during recovery from chilling. The differential acyl-editing of PA during recovery, which involves the remodelling of PC, might therefore be a regulatory mechanism of cold tolerance in barley.
在植物中，脂质代谢和重塑是在温度胁迫下生存的关键机制。本研究试图比较在冷胁迫处理下和随后恢复到胁迫状态下大麦根中的脂质谱。通过使用极性溶剂混合物的单一提取方法获得脂质，然后进行质谱分析。结果表明，脂质代谢受到冷胁迫的显著影响。在短期和长期恢复过程中，大多数分析的甘油磷脂（GL）都恢复到对照值，而几种代表性的磷脂酸（PA）分子物种在长期恢复过程中被编辑。在长期恢复过程中增加的大多数 PA 分子物种与减少的磷脂酰胆碱（PC）物种具有相同的酰基链。C34:2 和 C36:4 发生了最显著的变化。鉴于大麦根中 PC 酰基编辑的机制仍不清楚，我们还评估了溶血磷脂酰基转移酶（HvLPCAT）和磷脂酶 A（HvPLA）的贡献。与上述结果一致，HvLPCAT 和 HvPLA 基因在从冷胁迫中恢复时表达上调。因此，在恢复过程中 PA 的差异酰基编辑，涉及 PC 的重塑，可能是大麦耐寒性的一种调节机制。
