State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China; Research Institute of Standards and Norms, Ministry of Housing and Urban-Rural Development, Beijing 100835, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
Sci Total Environ. 2022 Jan 20;805:150086. doi: 10.1016/j.scitotenv.2021.150086. Epub 2021 Sep 1.
Heavy metals driven co-selection of antibiotic resistance in soil and water bodies has been widely concerned, but the response of antibiotic resistance to co-existence of antibiotics and heavy metals in composting system is still unknown. Commonly used sulfamethoxazole and copper were individually and jointly added into four reactors to explore their effects on antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), heavy metal resistance genes (MRGs) and bacterial community structure. The abundance of total ARGs and MGEs were notably decreased by 68.64%-84.95% and 91.27-97.38%, respectively, after the composting. Individual addition of sulfamethoxazole, individual addition of copper, simultaneously addition of sulfamethoxazole and copper increased the abundance of ARGs and MGEs throughout the composting period. Co-exposure of sulfamethoxazole and copper elevated the total abundance of ARGs by 1.17-1.51 times by the end of the composting compared to individual addition of sulfamethoxazole or copper. Network analysis indicated that the shifts in potential host bacteria determined the ARGs variation. Additionally, MGEs and MRGs had significant effects on ARGs, revealing that horizontal gene transfer and heavy metals induced co-resistance could promote ARGs dissemination.
重金属驱动的抗生素抗性在土壤和水体中的共同选择已受到广泛关注，但抗生素和重金属共存于堆肥系统中对抗生素抗性的响应仍不清楚。本研究采用常用的磺胺甲恶唑和铜单独及联合添加到四个反应器中，以研究其对抗生素抗性基因（ARGs）、移动遗传元件（MGEs）、重金属抗性基因（MRGs）和细菌群落结构的影响。在堆肥过程中，总 ARGs 和 MGEs 的丰度分别显著下降了 68.64%-84.95%和 91.27-97.38%。磺胺甲恶唑的单独添加、铜的单独添加、磺胺甲恶唑和铜的同时添加均在整个堆肥过程中增加了 ARGs 和 MGEs 的丰度。磺胺甲恶唑和铜的共暴露使堆肥结束时总 ARGs 的丰度比磺胺甲恶唑或铜的单独添加增加了 1.17-1.51 倍。网络分析表明，潜在宿主细菌的变化决定了 ARGs 的变化。此外，MGEs 和 MRGs 对抗生素基因有显著影响，表明水平基因转移和重金属诱导的共抗性可能促进了 ARGs 的传播。
