Paco-Larson M L, Nakanishi Y, Levine M, Garen A
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06511.
Dev Genet. 1986;7(4):197-203. doi: 10.1002/dvg.1020070405.
3H-labeled DNA probes for the ecdysterone-inducible Drosophila genes P1 and LSP-2 were hybridized in situ to RNA in sections of embryos and larvae. Intense hybridization was detected specifically in fat body cells of third-instar larvae and not in other cells of third-instar larvae nor in any cells at earlier stages. These results confirm the stringent tissue- and stage-specificity of P1 and LSP-2 expression. Hybridization of both probes occurred to virtually all the cells in the fat bodies, indicating that both genes are expressed in the same cells. Since P1 expression begins several hours later than LSP-2 expression, and appears to be induced directly by ecdysterone, this finding implies that one or more of the fat body components mediating the response to ecdysterone is gene-specific.
用于蜕皮激素诱导的果蝇基因P1和LSP-2的3H标记DNA探针，原位杂交到胚胎和幼虫切片中的RNA上。在三龄幼虫的脂肪体细胞中特异性地检测到强烈杂交信号，而在三龄幼虫的其他细胞中以及早期阶段的任何细胞中均未检测到。这些结果证实了P1和LSP-2表达具有严格的组织和阶段特异性。两种探针在脂肪体中的几乎所有细胞中都发生杂交，表明这两个基因在相同细胞中表达。由于P1表达比LSP-2表达晚数小时开始，并且似乎是由蜕皮激素直接诱导的，这一发现意味着介导对蜕皮激素反应的一种或多种脂肪体成分具有基因特异性。
