Roberts C T, Lasky S R, Lowe W L, Seaman W T, LeRoith D
Diabetes Branch, National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, Maryland 20892.
Mol Endocrinol. 1987 Mar;1(3):243-8. doi: 10.1210/mend-1-3-243.
Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe. The two types of rat IGF-I cDNA differed by the presence or absence of a 52-base pair insert which altered the derived C-terminal amino acid sequence of the E peptide, but not the 3'-untranslated region or the sequence coding for the mature IGF-I protein. When probes derived from these cDNA clones were hybridized to Northern blots of rat mRNA, specific bands of 8.6, 2.1, and 1.0-1.4 kilobases were seen. Hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF-I mRNA due to GH treatment in all tissues examined.
使用人胰岛素样生长因子I（IGF-I）cDNA探针，从成年大鼠肝脏文库中分离出两类IGF-I cDNA。这两类大鼠IGF-I cDNA的区别在于是否存在一个52个碱基对的插入片段，该插入片段改变了E肽的推导C末端氨基酸序列，但不影响3'-非翻译区或成熟IGF-I蛋白的编码序列。当将源自这些cDNA克隆的探针与大鼠mRNA的Northern印迹杂交时，观察到8.6、2.1和1.0 - 1.4千碱基的特异性条带。与生长激素处理组和对照组大鼠各种组织的聚腺苷酸加尾RNA（poly(A)+ RNA）杂交显示，在所有检测的组织中，生长激素处理均导致IGF-I mRNA增加。
