Zhang Guanglin, Fang Xiancun, Liu Chunlong, Han Fei
Department of Gastroenterology, Shouguang People's Hospital Shouguang, Shandong Province, China.
Department of General Surgery, Shouguang People's Hospital Shouguang, Shandong Province, China.
Am J Transl Res. 2021 Aug 15;13(8):9514-9521. eCollection 2021.
To evaluate the predictive value of circulating tumor cell count (CTC) and serum carcinoembryonic antigen (CEA) mRNA levels in postoperative recurrence of digestive tract cancer.
A total of 52 patients with digestive tract cancer who underwent radical resection in our hospital were divided into a recurrent group (n=18) and a non-recurrent group (n=34). The CTC and CEA mRNA levels of the two groups were compared one day before the operation. Pearson correlation analysis was used to analyze the correlation between CTC and CEA mRNA levels. The curve of subjects' working characteristics was used to analyze the predictive value of the serum CTC and the level of CEA mRNA in postoperative recurrence of digestive tract cancer. The correlation of CTC and CEA mRNA expression with different age, sex, cancer type and TNM stage was further analyzed.
CTC and CEA mRNA levels in the recurrent group were higher than those in the non-recurrent group (P<0.05). CTC was positively correlated with the CEA mRNA level by Pearson correlation (r=0.609, P=0.000). ROC curve showed that the area under the curve of CEA mRNA, CTC and combined test in predicting postoperative recurrence of digestive tract cancer were 0.912, 0.831 and 0.965 respectively. The area under the curve of the combined test and CEA mRNA level was significantly higher than that of CTC (Z=3.794, P=0.000; Z=2.001, P=0.045). There was no significant difference in the area under the curve between combined detection and CEA mRNA (Z=1.437 and P=0.151). There was no significant difference in CTC and CEA mRNA levels among patients with different sex, age, body weight, differentiation degree and histological type (P>0.05). The CTC and CEA mRNA levels of patients in stage lll and lV were higher than those in stage l and ll and the difference was statistically significant (P<0.05). By Spearman rank correlation analysis, it was found that CTC and CEA mRNA levels were positively correlated with the TNM stage (r=0.532, 0.712, P<0.05).
levels of CTC and serum CEA mRNA have a certain value in predicting postoperative recurrence of digestive tract cancer and their expression is closely related to TNM stage. Clinical diagnosis of both of them can improve the diagnostic efficiency of predicting postoperative recurrence.
评估循环肿瘤细胞计数（CTC）及血清癌胚抗原（CEA）mRNA水平对消化道癌术后复发的预测价值。
选取我院52例行根治性切除术的消化道癌患者，分为复发组（n = 18）和未复发组（n = 34）。比较两组术前1天的CTC及CEA mRNA水平。采用Pearson相关分析分析CTC与CEA mRNA水平的相关性。采用受试者工作特征曲线分析血清CTC及CEA mRNA水平对消化道癌术后复发的预测价值。进一步分析CTC及CEA mRNA表达与不同年龄、性别、癌症类型及TNM分期的相关性。
复发组的CTC及CEA mRNA水平高于未复发组（P < 0.05）。Pearson相关分析显示CTC与CEA mRNA水平呈正相关（r = 0.609，P = 0.000）。ROC曲线显示，CEA mRNA、CTC及联合检测预测消化道癌术后复发的曲线下面积分别为0.912、0.831和0.965。联合检测与CEA mRNA水平的曲线下面积显著高于CTC（Z = 3.794，P = 0.000；Z = 2.001，P = 0.045）。联合检测与CEA mRNA曲线下面积差异无统计学意义（Z = 1.437，P = 0.151）。不同性别、年龄、体重、分化程度及组织学类型患者的CTC及CEA mRNA水平差异无统计学意义（P > 0.05）。Ⅲ期和Ⅳ期患者的CTC及CEA mRNA水平高于Ⅰ期和Ⅱ期，差异有统计学意义（P < 0.05）。Spearman秩相关分析发现，CTC及CEA mRNA水平与TNM分期呈正相关（r = 0.532，0.712，P < 0.05）。
CTC及血清CEA mRNA水平对消化道癌术后复发有一定的预测价值，其表达与TNM分期密切相关。两者联合检测可提高预测术后复发的诊断效率。
