Dux E, Kloiber O, Hossmann K A, Siklós L
Max-Planck-Institute for Neurological Research, Department of Experimental Neurology, Cologne, FRG.
Acta Biol Hung. 1987;38(2):213-24.
The effect of lidocaine seizures on cellular accumulation of calcium was studied in hippocampal subfields CA1 and CA3 and the dentate gyrus of rats, using the combined oxalate-pyroantimonate method. The specificity of the reaction was ascertained by EGTA treatment and X-ray microanalysis. In control rats, calcium was visualized between myelin lamellae of axons, in synaptic vesicles and in some lysosomes. Two hours after onset of lidocaine seizures selective neuronal degenerations appeared in hippocampal subfields CA1 and CA3 but not in the dentate gyrus. Calcium deposits were present in numerous mitochondria of pyramidal cells and, occasionally, also of neuroglial cells. Many of these mitochondria exhibited ultrastructural alterations. Calcium uptake was most prominent in the CA3 sector but was also present in the CA1 subfield as well as the dentate gyrus. Intracellular calcium uptake, in consequence, is not the unique attribute of selectively vulnerable hippocampal neurons.
采用草酸-焦锑酸盐联合方法，研究利多卡因诱发的癫痫发作对大鼠海马CA1和CA3亚区以及齿状回细胞钙蓄积的影响。通过EGTA处理和X射线微分析确定反应的特异性。在对照大鼠中，钙可见于轴突的髓鞘板之间、突触小泡和一些溶酶体中。利多卡因诱发癫痫发作两小时后，海马CA1和CA3亚区出现选择性神经元变性，但齿状回未出现。钙沉积存在于锥体细胞的大量线粒体中，偶尔也存在于神经胶质细胞的线粒体中。许多这些线粒体表现出超微结构改变。钙摄取在CA3区最为显著，但在CA1亚区以及齿状回也存在。因此，细胞内钙摄取并非选择性易损海马神经元的独特特征。
