中性粒细胞胞外诱捕网形成的展示与定量分析
Demonstration and Quantification of Neutrophil Extracellular Trap Formation.
作者信息
Jiang Dongsheng, Saffarzadeh Mona, Scharffetter-Kochanek Karin
机构信息
Department of Dermatology and Allergic Diseases, University of Ulm, Ulm, Germany.
Department of Biochemistry, School of Medicine, Justus-Liebig-University of Giessen, Giessen, Germany.
出版信息
Bio Protoc. 2017 Jul 5;7(13):e2386. doi: 10.21769/BioProtoc.2386.
Abstract
In the recent decade, neutrophil extracellular traps (NETs) have been identified and confirmed as a new anti-microbial weapon of neutrophils. In this protocol, we describe easy methods to demonstrate NET formation by immunofluorescence staining of extracellular chromatin fiber with anti-DNA/Histone H1 antibody and quantification of NETs by using a non-cell-permeable DNA specific dye Sytox orange.
摘要
在最近十年中，中性粒细胞胞外陷阱（NETs）已被鉴定并确认为中性粒细胞的一种新型抗菌武器。在本方案中，我们描述了通过用抗DNA/组蛋白H1抗体对细胞外染色质纤维进行免疫荧光染色来证明NET形成的简便方法，以及使用非细胞渗透性DNA特异性染料Sytox orange对NETs进行定量的方法。
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