戊型肝炎病毒的RNA依赖性RNA聚合酶检测
RNA-dependent RNA Polymerase Assay for Hepatitis E Virus.
作者信息
Nair Vidya P, Anang Saumya, Srivastava Akriti, Surjit Milan
机构信息
Virology Laboratory, Vaccine and Infectious Disease Research Centre, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, Haryana, India.
出版信息
Bio Protoc. 2017 Apr 5;7(7):e2199. doi: 10.21769/BioProtoc.2199.
RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome. This assay measures activity of the hepatitis E virus (HEV) RdRp. In contrast to protocols available to assay the RdRp activity of many other viruses, this assay utilizes DIG-11-UTP as a nonradioactive alternative to P-UTP, thereby increasing the convenience of performing the assay.
RNA依赖性RNA聚合酶（RdRp）对于RNA病毒的病毒RNA复制至关重要。它合成病毒基因组RNA的互补链，随后该互补链用作模板以产生更多病毒基因组拷贝。此检测方法用于测量戊型肝炎病毒（HEV）RdRp的活性。与用于检测许多其他病毒RdRp活性的现有方案不同，该检测方法使用地高辛-11-UTP作为P-UTP的非放射性替代物，从而提高了进行该检测的便利性。
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