来自……的GanB半乳聚糖酶蛋白的半乳聚糖酶活性测定
Measurement of the Galactanase Activity of the GanB Galactanase Protein from .
作者信息
Watzlawick Hildegard
机构信息
Institut für Industrielle Genetik, Universität Stuttgart, Stuttgart, Germany.
出版信息
Bio Protoc. 2017 Apr 5;7(7):e2206. doi: 10.21769/BioProtoc.2206.
Abstract
The activity of the endo-β-1,4-galactanase GanB from on the high molecular weight β-1,4-galactan was determined quantitatively by the measurement of the increase of the reducing power or with the dyed substrate Azo-galactan. The generated degradation products were analyzed using thinlayer-chromatography (TLC) or high-performance anion-exchange chromatography (HPAEC).
摘要
通过测量还原力的增加或使用染色底物偶氮半乳聚糖，定量测定了[来源]的内切-β-1,4-半乳聚糖酶GanB对高分子量β-1,4-半乳聚糖的活性。使用薄层色谱（TLC）或高效阴离子交换色谱（HPAEC）分析产生的降解产物。
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