Wefers Christina, Bakdash Ghaith, Martin Meritxell Mereno, Boer Tjitske Duiveman-de, Torensma Ruurd, Massuger Leon F A G, de Vries Jolanda I M
Department of Obstetrics & Gynecology, Radboudumc, Nijmegen, The Netherlands.
Department of Tumor Immunology, Radboudumc, Nijmegen, The Netherlands.
Bio Protoc. 2017 Apr 5;7(7):e2219. doi: 10.21769/BioProtoc.2219.
Ovarian cancer is one of the most fatal tumors in women. Due to a lack of symptoms and adequate screening methods, patients are diagnosed at advanced stages with extensive tumor burden (Jelovac and Armstrong, 2011). Interestingly, ovarian cancer metastasis is generally found within the peritoneal cavity rather than other tissues (Lengyel, 2010; Tan , 2006 ). The reason behind this tissue tropism of the peritoneal cavity remains elusive. A prominent feature of this selectivity is ascites, the accumulation of fluid within the peritoneal cavity, containing, amongst others, immune cells, tumor cells and various soluble factors that can be involved in the progression of ovarian cancer ( Kipps , 2013 ). The protocol described here is used to isolate mononuclear cells from ascites to study the functionality of the immune system within the peritoneal cavity.
卵巢癌是女性最致命的肿瘤之一。由于缺乏症状和适当的筛查方法，患者在肿瘤负荷广泛的晚期才被诊断出来（杰洛瓦茨和阿姆斯特朗，2011年）。有趣的是，卵巢癌转移通常发生在腹腔内，而非其他组织（伦吉尔，2010年；谭，2006年）。这种腹腔组织嗜性背后的原因仍然不明。这种选择性的一个突出特征是腹水，即腹腔内液体的积聚，其中除其他物质外，还含有免疫细胞、肿瘤细胞和各种可能参与卵巢癌进展的可溶性因子（基普斯，2013年）。此处描述的方案用于从腹水中分离单核细胞，以研究腹腔内免疫系统的功能。
