Time-lapse Observation of Chromosomes, Cytoskeletons and Cell Organelles during Male Meiotic Divisions in .

In this protocol, we provide an experimental procedure that perform time-lapse observation of intra-cellular structures such as chromosomes, cytoskeletons and cell organelles during meiotic cell divisions in males. As primary spermatocyte is the largest dividing diploid cell in , which is equivalent in size to mammalian cultured cells, one can observe dynamics of cellular components during division of the model cells more precisely. Using this protocol, we have showed that a microtubule-associated protein plays an essential role in microtubule dynamics and initiation of cleavage furrowing through interaction between microtubules and actomyosin filaments. We have also reported that nuclear membrane components are required for a formation and/or maintenance of the spindle envelope essential for cytokinesis in the cells.