基于交配的分裂泛素检测法用于蛋白质相互作用检测
Mating Based Split-ubiquitin Assay for Detection of Protein Interactions.
作者信息
Horaruang Wijitra, Zhang Ben
机构信息
Laboratory of Plant Physiology and Biophysics, Bower Building, University of Glasgow, Glasgow, UK.
出版信息
Bio Protoc. 2017 May 5;7(9):e2258. doi: 10.21769/BioProtoc.2258.
Abstract
The mating based split-ubiquitin (mbSUS) assay is an alternative method to the classical yeast two-hybrid system with a number of advantages. The mbSUS assay relies on the ubiquitin-degradation pathway as a sensor for protein-protein interactions, and it is suitable for the determination of interactions between full-length proteins that are cytosolic or membrane-bound. Here we describe the mbSUS assay protocol which has been used for detecting the interaction between K channel and SNARE proteins ( Grefen , 2010 and 2015; Zhang , 2015 and 2016).
摘要
基于交配的分裂泛素（mbSUS）分析是经典酵母双杂交系统的一种替代方法，具有许多优点。mbSUS分析依赖泛素降解途径作为蛋白质-蛋白质相互作用的传感器，适用于确定胞质或膜结合全长蛋白质之间的相互作用。在这里，我们描述了已用于检测钾通道和SNARE蛋白之间相互作用的mbSUS分析方案（Grefen，2010年和2015年；Zhang，2015年和2016年）。
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