Horaruang Wijitra, Zhang Ben
Laboratory of Plant Physiology and Biophysics, Bower Building, University of Glasgow, Glasgow, UK.
Bio Protoc. 2017 May 5;7(9):e2258. doi: 10.21769/BioProtoc.2258.
The mating based split-ubiquitin (mbSUS) assay is an alternative method to the classical yeast two-hybrid system with a number of advantages. The mbSUS assay relies on the ubiquitin-degradation pathway as a sensor for protein-protein interactions, and it is suitable for the determination of interactions between full-length proteins that are cytosolic or membrane-bound. Here we describe the mbSUS assay protocol which has been used for detecting the interaction between K channel and SNARE proteins ( Grefen , 2010 and 2015; Zhang , 2015 and 2016).
基于交配的分裂泛素（mbSUS）分析是经典酵母双杂交系统的一种替代方法，具有许多优点。mbSUS分析依赖泛素降解途径作为蛋白质-蛋白质相互作用的传感器，适用于确定胞质或膜结合全长蛋白质之间的相互作用。在这里，我们描述了已用于检测钾通道和SNARE蛋白之间相互作用的mbSUS分析方案（Grefen，2010年和2015年；Zhang，2015年和2016年）。
