Chen Yong, Li Boqiang, Zhang Zhanquan, Tian Shiping
Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing, China.
Bio Protoc. 2017 May 5;7(9):e2264. doi: 10.21769/BioProtoc.2264.
, a widespread filamentous fungus, is a major causative agent of fruit decay and leads to huge economic losses during postharvest storage and shipping. Furthermore, it produces mycotoxin on the infected fruits that may cause harmful effects to human health. This pathogenicity assay involves a stab inoculation procedure of on apple fruit, an important experimental technique to study fungal pathogenesis. This assay can be applied to analyze the virulence of postharvest pathogen on other fruits such as orange, pear and kiwifruit.
，一种广泛存在的丝状真菌，是水果腐烂的主要致病因子，在采后储存和运输过程中会导致巨大的经济损失。此外，它会在受感染的水果上产生霉菌毒素，可能对人类健康造成有害影响。这种致病性测定涉及对苹果果实进行刺伤接种程序，这是研究真菌发病机制的一项重要实验技术。该测定可用于分析采后病原菌对其他水果如橙子、梨和猕猴桃的毒力。
