Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Center for Regenerative Medicine, National Center for Child Health and Development, 2-10-1 Ohkura, Setagaya-ku, Tokyo 157-8535, Japan.
STAR Protoc. 2021 Sep 11;2(3):100811. doi: 10.1016/j.xpro.2021.100811. eCollection 2021 Sep 17.
We recently demonstrated that the zygotic gene is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).
我们最近证明，合子基因在有丝分裂过程中参与维持多能干细胞（PSCs）的基因组完整性，并且该基因表达可以防止染色体异常。在这里，我们提供了一个详细的方案，使用 piggyBac 转座子基因表达载体系统，用组成型表达质粒转染小鼠胚胎干细胞（mESCs）和小鼠诱导多能干细胞（miPSCs）。有关此方案使用和执行的完整详细信息，请参见 Ogawa 等人。（2019 年）。
