Clin Lab. 2021 Sep 1;67(9). doi: 10.7754/Clin.Lab.2021.210117.
Preeclampsia (PE) is one of the most serious disorders of human pregnancy with a high rate of mortality for the fetus and mother. Several etiological factors are involved in the onset of this disease. Upregulation of IL-27 has been reported in placental tissue recovered from preeclamptic women, but the role of IL-27 has not yet been investigated in PE. The aim of the study was to investigate the association of IL-27 rs153109 and rs17855750 gene polymorphisms with PE; also, protein levels and susceptibility and severity of PE in Iranian women were evaluated.
This case-control study was performed on 199 PE patients and 228 healthy women as the control group. IL-27 rs153109 and rs17855750 SNPs were genotyped using a PCR-RFLP method. Moreover, the levels of IL-27 were determined in 40 PE and 45 healthy women using ELISA method.
Statistical analysis indicated that there were no differences in genotype, allele and genotype combination frequencies in the SNPs between cases and controls. The plasma level of IL-27 was elevated in the mild form of the disease compared with controls (p-value: 0.006).
The effect of IL-27 in preeclampsia is not due to the studied cytokine polymorphisms, but the level of IL-27 might be associated with the severity of preeclampsia in Iranian women.
子痫前期（PE）是人类妊娠中最严重的疾病之一，胎儿和母亲的死亡率都很高。这种疾病的发病涉及多个病因因素。据报道，在从患有子痫前期的女性中回收的胎盘组织中，IL-27 的表达上调，但 IL-27 在 PE 中的作用尚未得到研究。本研究旨在探讨 IL-27 rs153109 和 rs17855750 基因多态性与 PE 的相关性；还评估了伊朗女性中 IL-27 蛋白水平与 PE 的易感性和严重程度。
这项病例对照研究共纳入 199 例 PE 患者和 228 例健康女性作为对照组。采用 PCR-RFLP 方法检测 IL-27 rs153109 和 rs17855750 SNP。此外，采用 ELISA 法检测 40 例 PE 和 45 例健康女性的 IL-27 水平。
统计分析表明，病例组和对照组在 SNP 中的基因型、等位基因和基因型组合频率无差异。与对照组相比，疾病轻度患者的 IL-27 血浆水平升高（p 值：0.006）。
IL-27 在子痫前期中的作用不是由于研究的细胞因子多态性引起的，而是 IL-27 水平可能与伊朗女性子痫前期的严重程度有关。
