Thomsen P D, Christensen K
Acta Vet Scand. 1987;28(3-4):451-3. doi: 10.1186/BF03548615.
Recombinam DNA technologies offer new methods of detecting animal diversity at various levels. DNA »fingerprinting« using cloned repetitive sequences can be used to distinguish between individuals within breeds and may thus be used for paternity testing ( 1985). The forensic value of evidence of this kind has given guarded approval by the UK Immigration Advisory Service in its annual report ( 1987). At the between-species level labeled total genomic DNA was used by (1985) as probe in an in situ hybridization for speciesverification of chromosomal materials in human-rodent hybrid cell lines, and we recently described the use of H-labeled genomic pig DNA probe for detection af swine chromosomes in swine-hamster hybrid cell lines ( 1986).
重组DNA技术提供了在各个层面检测动物多样性的新方法。利用克隆的重复序列进行DNA“指纹识别”可用于区分同一品种内的个体,因此可用于亲子鉴定(1985年)。这类证据的法医价值已得到英国移民咨询服务机构在其年度报告(1987年)中的谨慎认可。在物种间层面,(1985年)使用标记的总基因组DNA作为探针,在原位杂交中对人-鼠杂种细胞系中的染色体物质进行物种鉴定,并且我们最近描述了使用H标记的基因组猪DNA探针来检测猪-仓鼠杂种细胞系中的猪染色体(1986年)。