Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Prague, Czech Republic.
Department of Analytical Chemistry, University of Chemistry and Technology Prague, Prague, Czech Republic.
J Sep Sci. 2021 Nov;44(22):4132-4140. doi: 10.1002/jssc.202100468. Epub 2021 Oct 3.
At present, Alzheimer's disease is detected mainly using psychological tests, which can only confirm the disease in its more advanced phases. Therefore, bioanalytical possibilities for detecting this disease earlier are being investigated. To date, the results of analyses, which focus mainly on the study of lipids and proteins either in cerebrospinal fluid or much less often in blood plasma, do not provide satisfactory results. In addition, cerebrospinal fluid sampling is uncomfortable for the patients and involves many health risks. In this work, we deal with proteomic analysis using Matrix-Assisted Laser Desorption/Ionisation-Time of Flight and Liquid Chromatography coupled to tandem Mass Spectrometry of blood plasma with a focus on various ways of preanalytical sample treatments. This should lead to results improvement and facilitate the subsequent evaluation using principal component analysis and partial least squares discriminant analysis. The obtained results indicate the direction of further research, namely the study of interactions between proteins and lipids contained in blood plasma. These substances may be regarded as potential biomarkers allowing for the diagnosis of Alzheimer´s disease even in its early stages.
目前，阿尔茨海默病主要通过心理测试来检测，但这种方法只能在疾病的更晚期阶段确认。因此，人们正在研究生物分析方法，以更早地发现这种疾病。迄今为止，这些分析主要集中在对脑脊液中的脂质和蛋白质，或者在较少情况下对血浆中的脂质和蛋白质的研究，其结果并不令人满意。此外，脑脊液采样对患者来说并不舒适，而且存在许多健康风险。在这项工作中，我们使用基质辅助激光解吸/电离飞行时间和液相色谱与串联质谱联用进行蛋白质组学分析，并专注于各种预处理方法。这应该会提高结果质量，并使用主成分分析和偏最小二乘判别分析来促进后续评估。所得结果表明了进一步研究的方向，即研究血浆中蛋白质和脂质之间的相互作用。这些物质可能被视为潜在的生物标志物，即使在疾病的早期阶段，也可以用于阿尔茨海默病的诊断。
