Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Department of Frontier Medical Science and Technology for Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Immun Inflamm Dis. 2021 Dec;9(4):1815-1819. doi: 10.1002/iid3.536. Epub 2021 Sep 21.
Some but not all patients with atopic dermatitis (AD) present with allergic conjunctival disease (ACD) including severe types such as atopic keratoconjunctivitis (AKC) with/without giant papillae. We hypothesized that different factors are involved in the severity of ACD and AD. Recently we reported that hsa-miR-628-3p could affect the balance of innate immunity by suppressing pathogen-associated molecular patterns such as toll-like receptor 3 (TLR3), RIG-I, and MDA-5. We also reported that TLR3 positively regulates ocular surface- and skin inflammation such as contact dermatitis and AD. Here we compared the plasma level of miR-628-3p in AD patients with severe AKC with giant papillae and/or shield ulcers, with the level in healthy controls and AD patient without AKC or with very mild AKC.
We used the plasma from 32 AD patients with severe AKC, from 40 healthy controls, and from 23 AD patient without AKC or with very mild AKC without giant papillae nor shield ulcers. Quantitative microRNA PCR assays were used to measure their plasma level of miR-628-3p.
We found that plasma miR-628-3p was upregulated in AD with severe AKC, but not in severe AD without severe AKC, nor in our healthy controls.
Our new findings suggest that the plasma miR-628-3p level may represent a marker to predict the presence of severe AKC in AD patients.
一些但不是所有特应性皮炎（AD）患者表现出过敏性结膜疾病（ACD），包括特应性角结膜炎（AKC）伴/不伴巨大乳头等严重类型。我们假设ACD 和 AD 的严重程度涉及不同的因素。最近我们报道 hsa-miR-628-3p 通过抑制模式识别受体（如 TLR3、RIG-I 和 MDA-5）等病原体相关分子模式，可以影响先天免疫的平衡。我们还报道 TLR3 可正向调节眼表和皮肤炎症，如接触性皮炎和 AD。在这里，我们比较了伴有巨大乳头和/或盾状溃疡的严重 AKC 的 AD 患者、健康对照者和无 AKC 或仅有非常轻微 AKC 且无巨大乳头或盾状溃疡的 AD 患者的血浆 miR-628-3p 水平。
我们使用了 32 例严重 AKC 的 AD 患者、40 名健康对照者和 23 名无 AKC 或仅有非常轻微 AKC 且无巨大乳头或盾状溃疡的 AD 患者的血浆，采用定量微 RNA PCR 检测来测量其血浆 miR-628-3p 水平。
我们发现严重 AKC 的 AD 患者血浆 miR-628-3p 上调，但严重 AKC 以外的严重 AD 患者以及健康对照者血浆 miR-628-3p 水平没有上调。
我们的新发现表明，血浆 miR-628-3p 水平可能是预测 AD 患者严重 AKC 存在的标志物。
