用于检测的种特异性 PCR 检测方法。

Species-specific PCR assay for the detection of .

机构信息

Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.

出版信息

J Vet Diagn Invest. 2021 Nov;33(6):1188-1192. doi: 10.1177/10406387211032927. Epub 2021 Sep 22.

DOI:10.1177/10406387211032927

PMID:34550025

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8546463/

Abstract

We developed a PCR assay for the detection of based on the 18S rRNA gene. Multiple specimens of were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other species and 2 species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of .

摘要

我们基于 18S rRNA 基因开发了一种用于检测的 PCR 检测方法。对多个的样本进行了检测，该检测方法始终产生一个小的、特异性的 306bp 的 PCR 产物。该 PCR 检测方法还与来自 10 种不同宿主物种的 13 种其他和 2 种物种的 DNA 进行了挑战；然而，观察到非特异性 DNA 扩增和多种带型，并且同一物种的不同分离株之间的扩增产物带型也不同。灵敏度确定为 6.4pg 的 DNA，估计寄生率为 0.0001%。该检测方法可用于的种特异性差异检测。