College of Dentistry, Mustansiriyah University, Iraq;
College of Dentistry, Mustansiriyah University, Iraq.
J Popul Ther Clin Pharmacol. 2021 Sep 15;28(1):63-72. doi: 10.47750/jptcp.2021.835. eCollection 2021.
Hypertrophic scars are pathological scars which result from exaggerated skin proliferation following a wound and injury. Although many theories have been implicated for keloidogenesis, the precise pathophysiological cause is still masked. Different treatment strategies have been tried in their management, but there is no satisfactory option for treating hypertrophic scar currently; moreover the standard steroid therapy is associated with numerous local side effects, and there is a need for researches in new treatment options. The aim of this study is to evaluate the role of topical isoxsuprine in experimentally induced hypertrophic scar in rabbits. In the current experimental study, 40 healthy male albino rabbits between 12 and 14 months of age were studied. These rabbits were categorized into five groups: healthy animal group ( = 8), hypertrophic scar without treatment (n = 8), hypertrophic scar treated with triamcinolone acetonide gel ( = 8), and hypertrophic scar treated with isoxsuprine gel ( = 8). Histological assessment of skin biopsy, including the conventional hematoxylin and eosin stain, and immunohistochemistry for transforming the growth factor beta 1 level (TGF-β1) and collagen 3 alpha1 (COLIIIαI) in skin tissue was done. The immunohistochemical score of TGF-β and collagen III was highest in group 2 (hypertrophic scar without treatment), followed by group 3 (hypertrophic scar treated with triamcinolone acetonide gel) and group 4 (hypertrophic scar treated with isoxsuprine gel) - no significant difference between them since > 0.05, and then by group 1 (healthy control group). Regarding histopathological scores of inflammation, the scar height, and scar index, the scores were highest in was highest in group 2 (hypertrophic scar without treatment), followed by group 3 (hypertrophic scar treated with triamcinolone acetonide gel) and group 4 (hypertrophic scar treated with isoxsuprine gel) - no significant difference between them since > 0.05, with the exception of index of scar, and then by group 1 (healthy control group). It was concluded that isouxoprine in a topical formulation greatly reduced inflammation and scar formation in deep wounds in a manner comparable to that seen with triamcinolone.
增生性瘢痕是一种病理性瘢痕，是由于伤口和损伤后皮肤过度增生而导致的。虽然有许多理论涉及瘢痕疙瘩的形成，但确切的病理生理原因仍不清楚。在其治疗中尝试了不同的治疗策略，但目前尚无令人满意的治疗增生性瘢痕的方法；此外，标准的类固醇治疗与许多局部副作用有关，因此需要研究新的治疗选择。本研究旨在评估局部异舒普林在兔实验性增生性瘢痕中的作用。在目前的实验研究中，研究了 40 只 12-14 月龄的健康雄性白化兔。这些兔子分为五组：健康动物组（ = 8）、未治疗的增生性瘢痕组（n = 8）、曲安奈德凝胶治疗的增生性瘢痕组（ = 8）和异舒普林凝胶治疗的增生性瘢痕组（ = 8）。对皮肤活检进行组织学评估，包括常规苏木精和伊红染色，以及转化生长因子β 1 水平（TGF-β1）和胶原蛋白 3α1（COLIIIαI）的免疫组织化学染色。免疫组织化学评分显示，TGF-β 和胶原 III 评分最高的是第 2 组（未治疗的增生性瘢痕），其次是第 3 组（曲安奈德凝胶治疗的增生性瘢痕）和第 4 组（异舒普林凝胶治疗的增生性瘢痕），差异无统计学意义（>0.05），然后是第 1 组（健康对照组）。关于炎症、瘢痕高度和瘢痕指数的组织病理学评分，第 2 组（未治疗的增生性瘢痕）最高，其次是第 3 组（曲安奈德凝胶治疗的增生性瘢痕）和第 4 组（异舒普林凝胶治疗的增生性瘢痕），差异无统计学意义（>0.05），但瘢痕指数除外，然后是第 1 组（健康对照组）。结论：局部异舒普林配方可显著减少深部伤口的炎症和瘢痕形成，其效果与曲安奈德相当。
