College of Engineering and Technology, Southwest University, Chongqing 400715, China.
Beijing Research Center for Information Technology in Agriculture, Beijing 100097, China.
Anal Methods. 2021 Sep 23;13(36):4120-4130. doi: 10.1039/d1ay00812a.
The detection of the wheat moisture content plays a key role in grain storage and classification. Harvested wheat grains were taken as samples in the current research. A total of 240 reaped wheat samples with different moisture contents were tested by applying terahertz (THz) spectroscopy. The frequency domain spectra and absorption coefficient spectra of wheat were obtained in the band of 0.1-1.2 THz, and the spectra were pretreated by mean centering, Savitzky-Golay (S-G), Multiplicative Scatter Correction (MSC) and Stand Normal Variate (SNV), respectively. Then a special algorithm of Tabu Search (TS) was used to find out the effective variables and remove the useless variables from the terahertz spectrum of the sample. Finally, the partial least squares (PLS) of chemometrics were used for quantitative model building and prediction. The correlation coefficient of calibration () is 0.9522. The root mean square error of calibration (RMSEC) is 0.4730. The correlation coefficient of prediction () is 0.9531. The root mean square error of prediction (RMSEP) is 0.5396. The results demonstrated that an accurate quantitative analysis of moisture in wheat samples could be achieved by terahertz time-domain spectroscopy combined with the TS algorithm. In addition, the results show that the model S-G + MSC + TS + PLS can effectively predict wheat moisture, and provide a rapid quantitative detection and analysis method for the detection of wheat moisture.
小麦水分含量的检测在粮食储存和分类中起着关键作用。本研究以收获的小麦籽粒为样品。应用太赫兹(THz)光谱技术对 240 个不同水分含量的收获小麦样本进行了测试。在 0.1-1.2 THz 波段获得了小麦的频域光谱和吸收系数光谱,并分别采用均值中心化、Savitzky-Golay(S-G)、多元散射校正(MSC)和标准正态变量(SNV)对光谱进行预处理。然后,采用禁忌搜索(TS)的特殊算法从样本的太赫兹光谱中找出有效变量并去除无用变量。最后,采用化学计量学的偏最小二乘(PLS)进行定量模型建立和预测。校正集的相关系数()为 0.9522。校正均方根误差(RMSEC)为 0.4730。预测集的相关系数()为 0.9531。预测均方根误差(RMSEP)为 0.5396。结果表明,太赫兹时域光谱结合 TS 算法可以实现对小麦样品水分的准确定量分析。此外,结果表明,S-G+MSC+TS+PLS 模型可以有效地预测小麦水分,为小麦水分的检测提供了一种快速定量检测和分析方法。