Tufan Elif, Sivas Güzin Göksun, Gürel-Gökmen Begüm, Yılmaz-Karaoğlu Sümeyye, Dursun Ercan, Çalışkan-Ak Esin, Muhan Aleyna, Özbeyli Dilek, Şener Göksel, Tunali-Akbay Tuğba
Marmara University, Faculty of Dentistry, Basic Medical Sciences, Biochemistry Department, İstanbul, Turkey.
Marmara University, Faculty of Dentistry, Basic Medical Sciences, Histology and Embryology Department, İstanbul, Turkey.
Br J Nutr. 2023 Nov 28;130(10):1704-1711. doi: 10.1017/S0007114523000752. Epub 2023 Mar 23.
Methotrexate (MTX) is a cytotoxic immunosuppressant that is widely used in the treatment of tumours, rheumatoid arthritis and psoriasis. This study aims to evaluate the effects of whey proteins on MTX-induced liver and kidney damage by focusing on oxidant–antioxidant systems and eating habits. The study was conducted in four groups of thirty Sprague–Dawley rats (control, control + whey protein concentrate (WPC), MTX, MTX + WPC). A single dose of 20 mg/kg MTX was administered intraperitoneally to the MTX groups. Control and MTX groups were given 2 g/kg WPC by oral gavage every day for 10 d. At the end of day 10, blood samples were drawn and liver and kidney tissues were removed. MTX administration increased the lipid peroxidation level and decreased glutathione level, superoxide dismutase and glutathione-S-transferase activities in the liver and kidney. Administration of WPC significantly reduced the damage caused by MTX in the liver and kidney. While a decrease in serum urea level and an increase in serum creatinine level were detected in the MTX group, WPC administration reversed these results up to control group levels. Administration of WPC to the MTX group significantly reversed the histopathological damage scores of the liver and kidney. WPC administration ameliorated the MTX-induced oxidative damage in the liver and kidney tissues due to its antioxidant properties. Liver and kidney damage can be prevented by using whey proteins as a nutraceutical in MTX therapy. In conclusion, whey proteins demonstrated a protective effect against MTX-induced liver and kidney damage.
甲氨蝶呤（MTX）是一种细胞毒性免疫抑制剂，广泛应用于肿瘤、类风湿性关节炎和银屑病的治疗。本研究旨在通过关注氧化-抗氧化系统和饮食习惯来评估乳清蛋白对MTX诱导的肝肾损伤的影响。该研究在四组30只Sprague-Dawley大鼠中进行（对照组、对照组+浓缩乳清蛋白（WPC）、MTX组、MTX+WPC组）。向MTX组腹腔注射单次剂量20mg/kg的MTX。对照组和MTX组每天经口灌胃给予2g/kg的WPC，持续10天。在第10天结束时，采集血样并取出肝肾组织。MTX给药增加了肝肾组织中的脂质过氧化水平，降低了谷胱甘肽水平、超氧化物歧化酶和谷胱甘肽-S-转移酶活性。WPC给药显著减轻了MTX对肝肾造成的损伤。虽然在MTX组中检测到血清尿素水平降低和血清肌酐水平升高，但WPC给药使这些结果恢复到对照组水平。向MTX组给予WPC显著逆转了肝肾的组织病理学损伤评分。由于其抗氧化特性，WPC给药改善了MTX诱导的肝肾组织氧化损伤。在MTX治疗中使用乳清蛋白作为营养保健品可以预防肝肾损伤。总之，乳清蛋白对MTX诱导的肝肾损伤具有保护作用。
