Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey.
Department of Biology, Faculty of Science, Ataturk University, 25240, Erzurum, Turkey.
Biol Futur. 2020 Jun;71(1-2):123-130. doi: 10.1007/s42977-020-00001-z.
Retrotransposon activity and genomic template stability (GTS) are one of the most significant rearranging mechanisms in environmental stress. Therefore, in this study, it is aimed to elucidate effecting of Cobalt (Co) on the instability of genomes and Long Terminal Repeat retrotransposon polymorphism in Zea mays and whether humic acid (HA) has any role on these parameters. For this purpose, Retrotransposon-microsatellite amplified polymorphism (REMAP) and Inter-Retrotransposon Amplified Polymorphism (IRAP) markers were applied to evaluate retrotransposon polymorphism and the GTS levels. It was found that IRAP and REMAP primers generate unique polymorphic band structures on maize plants treated with various doses of Co. Retrotransposon polymorphism increased and GTS decreased while increasing Co concentration. On the other hand, there was a reduction in negative effects of Co on retrotransposon GTS and polymorphism after treatment with HA. The results indicate that HA may be used effectively for the protection of maize seedlings from the destructive effects of Co toxicity.
逆转录转座子活性和基因组模板稳定性（GTS）是环境胁迫下最重要的重排机制之一。因此，本研究旨在阐明钴（Co）对基因组不稳定性的影响以及长末端重复逆转录转座子多态性在玉米中的作用，以及腐殖酸（HA）是否对这些参数有任何作用。为此，应用逆转录转座子-微卫星扩增多态性（REMAP）和内逆转录转座子扩增多态性（IRAP）标记来评估逆转录转座子多态性和 GTS 水平。结果发现，用不同剂量的 Co 处理玉米植株后，IRAP 和 REMAP 引物产生独特的多态性条带结构。随着 Co 浓度的增加，逆转录转座子多态性增加，GTS 降低。另一方面，HA 处理后，Co 对逆转录转座子 GTS 和多态性的负面影响降低。结果表明，HA 可有效用于保护玉米幼苗免受 Co 毒性的破坏作用。
