与栽培杨树（Populus）木材特性相关的候选基因中的等位基因变异。
Allelic variation in candidate genes associated with wood properties of cultivated poplars (Populus).
作者信息
Köbölkuti Zoltán Attila, Cseke Klára, Benke Attila, Báder Mátyás, Borovics Attila, Németh Róbert
机构信息
Department of Tree Breeding, National Agricultural Research and Innovation Centre, Forest Research Institute, Várkerulet 30/A, Sárvár, 9600, Hungary.
Simonyi Karoly Faculty of Engineering, Wood Sciences and Applied Arts, University of Sopron, Bajcsy Zs. u. 4, 9400, Sopron, Hungary.
出版信息
Biol Futur. 2019 Dec;70(4):286-294. doi: 10.1556/019.70.2019.32. Epub 2019 Dec 1.
INTRODUCTION
Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes.
MATERIALS AND METHODS
In this study, we addressed the need for a research methodology that initially identifies and then characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology.
RESULTS
On 23 different poplar species/hybrid samples, we successfully amplified 55 primers designed on Populus trichocarpa L. Considering the number of polymorphic sites, out of 73,206 bp, 51 SNPs and 31 indel events were found. Non-synonymous single base mutations could be detected in number of 30, 21 out of 164 sequences were the number of minimum recombination events and 41 significant pairwise comparisons between loci could be detected.
DISCUSSION AND CONCLUSION
Our results provide a roadmap for a future association genetic study between nucleotide diversity and precise evaluation of phenotype.
引言
由于杨树在木材、胶合板、纸浆和纸张方面具有实际价值，基因组研究应为进一步培育具有理想木材品质属性的杨树奠定坚实基础。
材料与方法
在本研究中，我们针对一种研究方法的需求展开探讨，该方法首先识别并随后表征编码具有木材特性表型性状的酶的候选基因，旨在开发基于基因组学的育种技术。
结果
在23个不同的杨树物种/杂交样本上，我们成功扩增了基于毛果杨设计的55条引物。考虑到多态性位点数量，在73206 bp中，发现了51个单核苷酸多态性（SNP）和31个插入缺失事件。在30个序列中可检测到非同义单碱基突变，164个序列中有21个是最小重组事件的数量，并且可以检测到41个位点之间的显著成对比较。
讨论与结论
我们的结果为未来核苷酸多样性与表型精确评估之间的关联遗传研究提供了路线图。
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