Graduate School, Jinan University, Jinan, People's Republic of China.
Department of Hematology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, People's Republic of China.
Hematology. 2021 Dec;26(1):716-723. doi: 10.1080/16078454.2021.1974199.
A previous study reported circ_0001821 serves as a new diagnostic biomarker in lung and gastric cancer. However, whether circ_0001821 associates with the development of multiple myeloma is still unclear.
To investigate the clinical significance and biological function of circ_0001821 in human multiple myeloma.
The expression of circ_0001821 in bone marrow tissues and cell lines of multiple myeloma was detected by quantitative real-time PCR. The relationship between circ_0001821 expression and clinicopathological characteristics in multiple myeloma patients was determined by the Chi-square test. MTT, Western blotting analysis and flow cytometry were performed to evaluate the impact of circ_0001821 on cell proliferation and apoptosis.
Circ_0001821 expression was increased in bone marrow tissues and cell lines from multiple myeloma compared with that in normal controls, and its expression was related to hemoglobin, bone injury, β2-MG, and globulin. In multiple myeloma patients aged ≥60 years, high circ_0001821 expression displayed lower overall survival (OS) rate compared with low circ_0001821 expression. High circ_0001821 expression was an independent prognostic indicator for the poor OS. Furthermore, the expression of Caspase-3 protein was lower in patients with high circ_0001821 expression than that in those patients with low circ_0001821expression. Overexpression of circ_0001821 led to increased proliferation and arrested apoptosis of multiple myeloma cells, while knockdown of circ_0001821 had the opposite effects.
High expression of circ_0001821 associated with aggressive pathological indicators and predicted a poor prognosis in multiple myeloma. Circ_0001821 played an oncogenic role in multiple myeloma by modulating cell proliferation and apoptosis.
先前的研究表明，circ_0001821 可作为肺癌和胃癌的新型诊断生物标志物。然而，circ_0001821 是否与多发性骨髓瘤的发生有关尚不清楚。
探讨 circ_0001821 在人类多发性骨髓瘤中的临床意义和生物学功能。
采用实时定量 PCR 检测多发性骨髓瘤患者骨髓组织和细胞系中 circ_0001821 的表达。采用卡方检验分析 circ_0001821 表达与多发性骨髓瘤患者临床病理特征的关系。采用 MTT、Western blot 分析和流式细胞术评估 circ_0001821 对细胞增殖和凋亡的影响。
与正常对照相比，多发性骨髓瘤患者骨髓组织和细胞系中 circ_0001821 的表达升高，且其表达与血红蛋白、骨损伤、β2-MG 和球蛋白有关。在年龄≥60 岁的多发性骨髓瘤患者中，高 circ_0001821 表达患者的总生存率（OS）低于低 circ_0001821 表达患者。高 circ_0001821 表达是 OS 不良的独立预后因素。此外，高 circ_0001821 表达患者的 Caspase-3 蛋白表达低于低 circ_0001821 表达患者。circ_0001821 过表达导致多发性骨髓瘤细胞增殖增加和凋亡阻滞，而 circ_0001821 敲低则产生相反的效果。
circ_0001821 高表达与侵袭性病理指标相关，并预示多发性骨髓瘤预后不良。circ_0001821 通过调节细胞增殖和凋亡在多发性骨髓瘤中发挥致癌作用。
