大鼠脑来源的突触体和质膜囊泡对酪氨酸摄取的发育研究。甲状腺激素的作用。

Developmental studies on the uptake of tyrosine by synaptosomes and plasma membrane vesicles derived from rat brain. Effect of thyroid hormones.

作者信息

Herrero E, Diez-Guerra J, Aragon M C, Valdivieso F, Gimenez C

机构信息

Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias, Universidad Autónoma, Madrid, Spain.

出版信息

Int J Dev Neurosci. 1986;4(1):13-20. doi: 10.1016/0736-5748(86)90011-0.

DOI:10.1016/0736-5748(86)90011-0

PMID:3455570

Abstract

The uptake of L-tyrosine at various stages of development was examined in synaptosomes and in plasma membrane vesicles derived from rat brain. The total uptake has two components, Na+-dependent and Na+-independent, respectively. The Na+-dependent component of the transport system appears around the 5th postnatal day and increases with age. The affinity of the transport system for tyrosine does not vary substantially during development. The Vmax increases more than six-fold between day 15 and adulthood. Plasma membrane vesicles derived from T3-treated rats accumulate more tyrosine than those obtained from the control animals. The results support the view that thyroid hormones during development promote the establishment of the systems implicated in neurotransmission in the developing nervous system.

摘要

在源自大鼠脑的突触体和质膜囊泡中，研究了L-酪氨酸在不同发育阶段的摄取情况。总摄取量分别有两个成分，即钠依赖性和非钠依赖性。转运系统的钠依赖性成分在出生后第5天左右出现，并随年龄增长而增加。在发育过程中，转运系统对酪氨酸的亲和力没有显著变化。最大反应速度（Vmax）在出生后第15天到成年期之间增加了六倍多。来自经三碘甲状腺原氨酸（T3）处理的大鼠的质膜囊泡比来自对照动物的囊泡积累更多的酪氨酸。这些结果支持了这样一种观点，即发育过程中的甲状腺激素促进了发育中的神经系统中与神经传递相关系统的建立。