基于紫外光辅助刻蚀金纳米棒的碱性磷酸酶的多色和光热双模式检测。
Multicolor and photothermal dual-mode assay of alkaline phosphatase based on the UV light-assisted etching of gold nanorods.
机构信息
School of Chemistry and Pharmaceutical Science, State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, 541004, PR China.
School of Chemistry and Pharmaceutical Science, State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, 541004, PR China.
出版信息
Anal Chim Acta. 2021 Oct 9;1181:338926. doi: 10.1016/j.aca.2021.338926. Epub 2021 Aug 9.
A multicolor and photothermal dual-mode assay for sensitive alkaline phosphatase (ALP) determination was realized based on the 3,3',5,5'-tetramethylbenzidine (TMB)-induced etching of gold nanorods (AuNRs). TMB was oxidized under ultraviolet light irradiation to form TMB. In the presence of ALP, ascorbic acid phosphate (AAP) is converted to ascorbic acid, which can then reduce the levels of TMB, resulting in lower concentrations of TMB. The remaining TMB was transformed into TMB after the addition of HCl solution. AuNRs were etched by TMB to produce a multicolor and photothermal change. Based on the degree of AuNRs etching, this highly sensitive dual-mode assay provided a linear range of 1.0-8.0 mU/mL, with detection limits of 0.34 mU/mL for the multicolor assay and 0.11 mU/mL for the photothermal assay. This method was successfully applied to the determination of ALP in serum samples.
一种基于 3,3',5,5'-四甲基联苯胺（TMB）诱导金纳米棒（AuNRs）刻蚀的多色和光热双模式测定碱性磷酸酶（ALP）的方法。TMB 在紫外光照射下被氧化形成 TMB。在碱性磷酸酶存在的情况下，磷酸抗坏血酸（AAP）被转化为抗坏血酸，从而降低 TMB 的水平，导致 TMB 的浓度降低。加入盐酸溶液后，剩余的 TMB 转化为 TMB。AuNRs 被 TMB 刻蚀产生多色和光热变化。基于 AuNRs 刻蚀的程度，这种高灵敏度的双模式测定法提供了 1.0-8.0 mU/mL 的线性范围，多色测定法的检测限为 0.34 mU/mL，光热测定法的检测限为 0.11 mU/mL。该方法成功应用于血清样品中 ALP 的测定。
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