Siebzehnrubl Florian A
European Cancer Stem Cell Research Institute, Cardiff University School of Biosciences, Cardiff, UK.
Methods Mol Biol. 2022;2389:95-102. doi: 10.1007/978-1-0716-1783-0_9.
Adult neural precursor cells are an integral part of the brain and have been a focus of intense research for half a century. Even though adult neural stem/progenitor cells in the human brain have been identified over 20 years ago, progress in this area has been slow, and the existence of lifelong neurogenesis in humans is still debated. Remarkable species differences exist between humans and rodents in astrocyte development and diversity, suggesting similar differences may exist in neural stem/progenitor cells and underscoring the need for further research in human tissue.This chapter provides a guideline for dissociation of adult human brain tissue from biopsy or autopsy specimens. While protocols for subsequent culturing of neural precursors are included, the main focus is the preparation of a suspension of viable single cells that may also be useful in other experimental paradigms, such as genomic profiling.
成体神经前体细胞是大脑不可或缺的一部分，半个世纪以来一直是深入研究的焦点。尽管人类大脑中的成体神经干细胞/祖细胞在20多年前就已被发现，但该领域的进展一直很缓慢，人类终身神经发生的存在仍存在争议。人类和啮齿动物在星形胶质细胞发育和多样性方面存在显著的物种差异，这表明神经干细胞/祖细胞可能也存在类似差异，凸显了对人类组织进行进一步研究的必要性。本章提供了从活检或尸检标本中分离成人脑组织的指南。虽然包括了后续培养神经前体细胞的方案，但主要重点是制备有活力的单细胞悬液，这在其他实验范式中也可能有用，例如基因组分析。
