Dong Wen-Qing, Miao Hua-Chun, Wu Feng, Li Huai-Bin
Department of Anatomy, Wannan Medical College, Wuhu 241002, Anhui Province, China.
Zhen Ci Yan Jiu. 2021 Sep 25;46(9):721-7. doi: 10.13702/j.1000-0607.20210187.
To observe the effect of electroacupuncture(EA)on the expression of myelin basic protein (MBP), axon growth inhibitor Nogo-A and Nogo receptor (NgR) in corpus callosum of rats with focal cerebral ischemia, so as to explore the mechanism of EA underlying improving ischemic white matter injury.
Fourty male SD rats were randomly divided into normal, sham operation, model and EA groups, with 10 rats in each group. The focal cerebral ischemia rat model was established by occlusion of the middle cerebral artery (MCAO). EA was applied to "Baihui"(GV20) and "Zusanli"(ST36) on the left side for 30 min, once daily for 14 days. Neurological function score and the adhensive removal test were used to evaluate neurological deficit severity; Hematoxylin-esion staining was used to observe the pathological changes in myelin of corpus callosum and luxol fast blue（LFB） staining was used to observe the myelin of corpus callosum. Immunohistochemical staining and Western blot were used to detect the expressions of MBP、Nogo-A and NgR in the ischemic corpus callosum.
After MCAO, the neurological function score was significantly increased (<0.05), the time required for contact with tape and tape removal was longer (<0.001), the intensity of LFB staining and the expression of MBP decreased, while the veside area and the expression of Nogo-A and its receptor NgR increased (<0.01, <0.05) in the model group relevant to the normal and sham operation groups. The fiber arrangement of the corpus callosum on the ischemic side was disordered and a large amount of myelin sheath was lost in the model group. Following the treatment, the neurological deficit score of EA group was gradually decreased and significantly decreased on the 3, 7 and 14 day (<0.05), and the time to remove the adhesive tape was shortened at the 7 and 14 day (<0.001). The shape of the corpus callosum in the EA group was close to normal, and the myelin structure was relatively complete. The intensity of LFB staining and the expression of MBP was increased (<0.05, <0.01) while the expression of Nogo-A and its receptor NgR were decreased in the EA group relevant to the model group (<0.01).
EA can play a protective role in myelin of the corpus callosum after cerebral ischemia, which may be related to down-regulating the expressions of Nogo-A and NgR.
观察电针（EA）对局灶性脑缺血大鼠胼胝体中髓鞘碱性蛋白（MBP）、轴突生长抑制因子Nogo - A和Nogo受体（NgR）表达的影响，以探讨电针改善缺血性白质损伤的机制。
40只雄性SD大鼠随机分为正常组、假手术组、模型组和电针组，每组10只。采用大脑中动脉闭塞法（MCAO）建立局灶性脑缺血大鼠模型。电针刺激左侧“百会”（GV20）和“足三里”（ST36），每次30分钟，每日1次，共14天。采用神经功能评分和黏附去除试验评估神经功能缺损严重程度；苏木精 - 伊红染色观察胼胝体髓鞘病理变化，Luxol固蓝（LFB）染色观察胼胝体髓鞘情况。免疫组织化学染色和蛋白质印迹法检测缺血胼胝体中MBP、Nogo - A和NgR的表达。
与正常组和假手术组相比，MCAO术后模型组神经功能评分显著升高（<0.05），接触胶带和去除胶带所需时间延长（<0.001），LFB染色强度和MBP表达降低，而空泡面积以及Nogo - A及其受体NgR表达增加（<0.01，<0.05）。模型组缺血侧胼胝体纤维排列紊乱，大量髓鞘丢失。治疗后，电针组神经功能缺损评分逐渐降低，在第3、7和14天显著降低（<0.05），第7和14天去除胶带时间缩短（<0.001）。电针组胼胝体形态接近正常，髓鞘结构相对完整。与模型组相比，电针组LFB染色强度和MBP表达增加（<0.05，<0.01），而Nogo - A及其受体NgR表达降低（<0.01）。
电针可对脑缺血后胼胝体髓鞘起到保护作用，这可能与下调Nogo - A和NgR的表达有关。
