Polho Gabriel B, Cardillo Giancarlo M, Kerr Daniel S, Chile Thais, Gattaz Wagner F, Forlenza Orestes V, Brentani Helena P, De-Paula Vanessa J
Laboratório de Neurociências (LIM-27), Departamento e Instituto de Psiquiatria, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, SP, Brasil.
Laboratório de Psicobiologia (LIM-23), Departamento e Instituto de Psiquiatria, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, SP, Brasil.
Neural Regen Res. 2022 May;17(5):1156-1160. doi: 10.4103/1673-5374.324852.
Antipsychotics may prolong or retain telomere length, affect mitochondrial function, and then affect the metabolism of nerve cells. To validate the hypothesis that antipsychotics can prolong telomere length after oxidative stress injury, leukocytes from healthy volunteers were extracted using Ficoll-Histopaque density gradient. The mononuclear cells layer was resuspended in cell culture medium. Oxidative stress was induced with hydrogen peroxide in cultured leukocytes. Four days later, leukocytes were treated with aripiprazole, haloperidol or clozapine for 7 days. Real-time PCR revealed that treatments with aripiprazole and haloperidol increased the telomere length by 23% and 20% in peripheral blood mononuclear cells after acute oxidative stress injury. These results suggest that haloperidol and aripiprazole can reduce the damage to telomeres induced by oxidative stress. The experiment procedure was approved by the Ethics Committee of Faculty of Medicine of the University of São Paulo (FMUSP/CAAE approval No. 52622616.8.0000.0065).
抗精神病药物可能会延长或维持端粒长度，影响线粒体功能，进而影响神经细胞的代谢。为验证抗精神病药物在氧化应激损伤后可延长端粒长度这一假说，采用Ficoll-Histopaque密度梯度法从健康志愿者中提取白细胞。将单核细胞层重悬于细胞培养基中。在培养的白细胞中用过氧化氢诱导氧化应激。四天后，用阿立哌唑、氟哌啶醇或氯氮平处理白细胞7天。实时聚合酶链反应显示，急性氧化应激损伤后，阿立哌唑和氟哌啶醇处理可使外周血单核细胞中端粒长度分别增加23%和20%。这些结果表明，氟哌啶醇和阿立哌唑可减轻氧化应激诱导的端粒损伤。实验程序经圣保罗大学医学院伦理委员会批准（FMUSP/CAAE批准号：52622616.8.0000.0065）。
