Hosari E, Brüderlein S, Gebhart E
Cancer Genet Cytogenet. 1986 Feb 1;20(1-2):63-71. doi: 10.1016/0165-4608(86)90108-1.
Micronucleation was induced by vincristine, colcemid, and colcemid in combination with cytochalasin B in cells of a human metastatic breast carcinoma cell line (MDA MB 231). Cells treated with the latter combination were enucleated subsequently by centrifugation in the presence of cytochalasin B. The resulting "microcell" fraction was fused with mitotic human primary fibroblasts or mitotic HeLa cells using polyethyleneglycol (PEG). The success of the microcell-mediated chromosome transfer thus could be demonstrated as premature condensation in the mitotic recipient of the transferred micronuclei. This technique of cytogenetic analysis allowed a fast and simple control of the influence of different conditions on micronucleation and fusion of micronuclei with recipient cells. It could be shown that microcell-mediated chromosome transfer from human tumor cells into human normal, as well as human tumor, recipient cells is practicable if the techniques figured out by the present study are employed.
用长春新碱、秋水仙酰胺以及秋水仙酰胺与细胞松弛素B联合处理人转移性乳腺癌细胞系（MDA MB 231）的细胞，诱导其微核形成。用后一种联合处理的细胞随后在细胞松弛素B存在的情况下通过离心去核。所得的“微细胞”部分使用聚乙二醇（PEG）与有丝分裂期的人原代成纤维细胞或有丝分裂期的HeLa细胞融合。微细胞介导的染色体转移的成功可表现为转移的微核在有丝分裂期受体细胞中过早凝聚。这种细胞遗传学分析技术能够快速、简单地控制不同条件对微核形成以及微核与受体细胞融合的影响。结果表明，如果采用本研究确定的技术，将人肿瘤细胞中的微细胞介导的染色体转移到人正常受体细胞以及人肿瘤受体细胞中是可行的。
